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Recombinant human HVEM / TNFRSF14 Protein, Strep Tag, 25µg  

Recombinant human HVEM / TNFRSF14 Protein, Strep Tag, 25µg

Recombinant Human HVEM Protein, With C-Twin Strep Tag (rh HVEM,twin strep tag) Leu 39 - Val 202 was produced in human 293 cells (HEK293).

Synonym: Recombinant, human, protein TNFRSF14,ATAR,HVEA,HVEM,CD270

More details

HVM-H5283-025

Availability: within 7 days

312,00 €

Background
Herpesvirus entry mediator (HVEM) is also known as TNFRSF14, TR2 (TNF receptorlike molecule) and ATAR (another TRAF associated receptor), is a type I membrane protein belonging to the TNF/NGF receptor superfamily. HVEM expression has been detected in peripheral blood T cells, B cells, monocytes and in various tissues enriched in lymphoid cells. The extracellular domain of HVEM has been shown to interact directly with the herpes simplex virus envelope glycoprotein D (gD). Two TNF superfamily ligands, including the secreted TNFβ (lymphotoxin α) and the membrane protein LIGHT (lymphotoxins, exhibits inducible expression, and competes with HSV glycoprotein D for HVEM, a receptor expressed by T lymphocytes), have been shown to be the cellular ligands for HVEM. Besides HVEM, LIGHT can also interact with LTβR, the receptor for lymphotoxin αβ heterotrimer. The role of the HVEM LIGHT /LTβ receptor ligand pair in immune function and herpesvirus pathobiology remains to be elucidated.

Source
Recombinant Human HVEM, Strep Tag (HVM-H5283) is expressed from human 293 cells (HEK293). It contains AA Leu 39 - Val 202 (Accession # NP_003811.2).
Predicted N-terminus: Leu 39

Molecular Characterization
This protein carries a twin strep tag at the C-terminus.
The protein has a calculated MW of 20.4 kDa. The protein migrates as 27-50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>90% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.

Bioactivity
Please refer to product data sheet.

Clinical and Translational Updates

(1) Hsu H., et al., 1997, J. Biol. Chem., 272:13471.
(2) Mauri, D.N. et al., 1998, Immunity 8:21.
(3) Montgomery, R.I. et al., 1996, Cell 87:427.
(4) Cheung,T.C.et al., 2005, Proc.Natl.Acad.Sci.USA102:13218-23.