Biaffin

Newsletter

kinases protein kinase inhibitors signaling activators kinase substrates antibodies bioluminescence assays
Recombinant, human pyridoxal kinase PDXK, 10 µg  

Recombinant, human pyridoxal kinase PDXK, 10 µg

PDXK (pyridoxal kinase), human recombinant and active enzyme

Alternate names: recombinant, human, kinase, Pyridoxine kinase, PDXK

More details

PK-PDXK-010

Availability: on stock

295,00 €

Background: Pyridoxal kinase is an enzyme involved in vitamin B6 metabolism. Since mammals cannot synthesize pyridoxal-5-phosphate (PLP), a cofactor for enzymes primarily involved in amino acids and sugar metabolism, dietary precursors such as pyridoxal, pyridoxamine and pyridoxine (classified collectively as vitamin B6) are essential. PDXK catalyzes the phosphorylation of vitamin B6 in the presence of ATP and divalent cations (Mg2+, Zn2+). In recent studies it becomes evident that genetic variations of PDXK are associated with Parkinson disease, since PLP is involved in the biosynthesis of dopamine, a neurotransmitter linked to the disease characteristics such as motor and movement disorders.

Protein concentration (Bradford with BSA as standard):        1. mg/ml
Purity:                                                                                        > 90% by SDS PAGE
Specific activity:                                                                       not determined
Molecular weight:                                                                     36 kDa

Ordering information: shipped on dry ice

Product specific literature:

Tang L, Li MH, Cao P, Wang F, Chang WR, Bach S, Reinhardt J, Ferandin Y, Galons H, Wan Y, Gray N, Meijer L, Jiang T, Liang DC. (2005) "Crystal structure of pyridoxal kinase in complex with roscovitine and derivatives." J Biol Chem. 280(35):31220-9.

Hanna MC, Turner AJ, Kirkness EF. (1997) "Human pyridoxal kinase. cDNA cloning, expression, and modulation by ligands of the benzodiazepine receptor." J Biol Chem. 18;272(16):10756-60.

Elstner M, Morris CM, Heim K, Lichtner P, Bender A, Mehta D, Schulte C, Sharma M, Hudson G, Goldwurm S, Giovanetti A, Zeviani M, Burn DJ, McKeith IG, Perry RH, Jaros E, Kr�ger R, Wichmann HE, Schreiber S, Campbell H, Wilson JF, Wright AF, Dunlop M, Pistis G, Toniolo D, Chinnery PF, Gasser T, Klopstock T, Meitinger T, Prokisch H, Turnbull DM. (2009) "Single-cell expression profiling of dopaminergic neurons combined with association analysis identifies pyridoxal kinase as Parkinson\'s disease gene." Ann Neurol. 66(6):792-8.