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Recombinant, human 5' AMP-activated protein kinase / AMPK alpha1, active kinase, 10 µg  

Recombinant, human 5' AMP-activated protein kinase / AMPK alpha1, active kinase, 10 µg

Recombinant human AMPK-alpha1, protein kinase

Alternate names: AMPKa1, AMPK subunit alpha, PRKAA1, AMPK a1, 5'-AMP-activated protein kinase, catalytic alpha-1, 5' adenosine monophosphate-activated protein kinase

More details

PK-AMPKA1-A010

Availability: within 3 days

325,00 €

5' AMP-activated protein kinase / AMPK or 5' adenosine monophosphate-activated protein kinase is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. 5'-AMP-activated protein kinase catalytic subunit alpha-1 is an enzyme that in humans is encoded by the PRKAA1 gene.

Human AMPK-alpha1, Amino acids M1-Q550 , N-terminally fused to GST-HIS6-Thrombin cleavage site

Theoretical MW: 92.188 kDa (fusion protein)
Expression system: Baculovirus infected Sf9 cells
Storage buffer: 50 mM Tris-HCl, pH 8.0; 100 mM NaCl, 5 mM DTT, 4 mM reduced glutathione, 20% glycerol
Protein concentration: 0.144 mg/ml (Bradford method using BSA as standard protein)
Method for determination of Km value & specific activity: Filter binding assay MSPH membrane
Specific activity: 37,000 pmol/mg x min

Entrez Gene ID: 5562
UniProtKB: Q13131

Ordering informations: shipped on dry ice

Product specific literature

Hardie DG (2007) "Annu Rev Pharmacol Toxicol." AMP-activated protein kinase as a drug target" 47:185-210.