Recombinant, human B lymphocyte kinase / BLK , active protein kinase, 10 µg

Background: B lymphocyte kinase (BLK) is a non-receptor tyrosine kinase involved in B-lymphocyte development, differentiation and signaling. Signaling through BLK plays an important role in transmitting signals through surface immunoglobulins and supports the pro-B to pre-B transition, as well as the signaling for growth arrest and apoptosis downstream of B-cell receptor. BLK specifically binds and phosphorylates CD79A, as well as CD79B, phosphorylates the immunoglobulin G receptors FCGR2A, FCGR2B and FCGR2C and plays together with FYN and LYN an essential role in pre-B-cell receptor (pre-BCR)-mediated NF-kappa-B activation.

Recombinant human B lymphocyte tyrosine kinase,  amino acids M1-P505 active enzyme, N-terminally fused to GST-HIS6-Thrombin cleavage site

Theoretical MW: 87.102 kDa (fusion protein)
Expression system: Baculovirus infected Sf9 cells
Storage buffer: 50 mM Tris-HCl, pH 8.0;100 mM NaCl, 5 mM DTT, 4 mM reduced glutathione, 20% glycerol
Protein concentration: 0.120 mg/ml (Bradford method using BSA as standard protein)
Method for determination of Km value & specific activity: Filter binding assay MSFC membrane
Specific activity: 167,000 pmol/mg x min

Entrez Gene ID: 640
UniProtKB: P51451

Ordering informations: shipped on dry ice

Product specific literature

Bewarder N., Weinrich V., Budde P., Hartmann D., Flaswinkel H., Reth M., Frey J. (1996) "In vivo and in vitro specificity of protein tyrosine kinases for immunoglobulin G receptor (FcgammaRII) phosphorylation." Mol. Cell. Biol. 16:4735-4743

Borowiec M., Liew C.W., Thompson R., Boonyasrisawat W., Hu J., Mlynarski W.M., El Khattabi I., Kim S.H., Marselli L., Rich S.S., Krolewski A.S., Bonner-Weir S., Sharma A., Sale M., Mychaleckyj J.C., Kulkarni R.N., Doria A. (2009) "Mutations at the BLK locus linked to maturity onset diabetes of the young and beta-cell dysfunction." Proc. Natl. Acad. Sci. U.S.A. 106:14460-14465