Recombinant, human breast tumor kinase / BRK, active protein kinase, 10 µg

Background: Breast tumor kinase (BRK) / PTK6 promotes cellular differentiation and apoptosis. In tumors PTK6 contributes to cancer progression by sensitizing cells to mitogenic signals and enhancing proliferation, anchorage-independent survival and migration/invasion. Association with EGFR, ERBB2, ERBB3 may contribute to mammary tumor development and growth through enhancement of EGF-induced signaling via BTK/AKT and PI3 kinase. PTK6 / BRK activates STAT3 and STAT5B to promote proliferation. Nuclear BRK/ PTK6 may be important for regulating growth in normal epithelia, while cytoplasmic PTK6 might activate oncogenic signaling pathways.

Recombinant human breast tumor kinase/tyrosine-protein kinase 6, Amino acids M1-T451, active enzmye, N-terminally fused to GST-HIS6-Thrombin cleavage site

Theoretical MW: 81.79 kDa (fusion protein)
Expression system: Baculovirus infected Sf9 cells
Storage buffer: 50 mM Tris-HCl, pH 8.0;100 mM NaCl, 5 mM DTT, 4 mM reduced glutathione, 20% glycerol
Protein concentration: 0.160 mg/ml (Bradford method using BSA as standard protein)
Method for determination of Km value & specific activity: Filter binding assay MAFC membrane
Specific activity: 31,000 pmol/mg x min

Entrez Gene ID: 5753
UniProtKB: Q13882

Ordering informations: shipped on dry ice

Product specific literature

Mitchell P.J., Sara E.A., Crompton M.R. (2000) "A novel adaptor-like protein which is a substrate for the non-receptor tyrosine kinase, BRK." Oncogene 19:4273-4282

Haegebarth A., Heap D., Bie W., Derry J.J., Richard S., Tyner A.L. (2004) "The nuclear tyrosine kinase BRK/Sik phosphorylates and inhibits the RNA-binding activities of the Sam68-like mammalian proteins SLM-1 and SLM-2." J. Biol. Chem. 279:54398-54404

Chen H.Y., Shen C.H., Tsai Y.T., Lin F.C., Huang Y.P., Chen R.H. (2004) "Brk activates rac1 and promotes cell migration and invasion by phosphorylating paxillin." Mol. Cell. Biol. 24:10558-10572

Zhang P., Ostrander J.H., Faivre E.J., Olsen A., Fitzsimmons D., Lange C.A. (2005) "Regulated association of protein kinase B/Akt with breast tumor kinase." J. Biol. Chem. 280:1982-1991

Lukong K.E., Larocque D., Tyner A.L., Richard S.  (2005) "Tyrosine phosphorylation of sam68 by breast tumor kinase regulates intranuclear localization and cell cycle progression." J. Biol. Chem. 280:38639-38647

Liu L., Gao Y., Qiu H., Miller W.T., Poli V., Reich N.C. (2006) "Identification of STAT3 as a specific substrate of breast tumor kinase." Oncogene 25:4904-4912

Weaver A.M., Silva C.M. (2007) "Signal transducer and activator of transcription 5b: a new target of breast tumor kinase/protein tyrosine kinase 6." Breast Cancer Res. 9:R79-R79

Shen C.H., Chen H.Y., Lin M.S., Li F.Y., Chang C.C., Kuo M.L., Settleman J., Chen R.H. (2008) "Breast tumor kinase phosphorylates p190RhoGAP to regulate rho and ras and promote breast carcinoma growth, migration, and invasion." Cancer Res. 68:7779-7787

Brauer P.M., Zheng Y., Evans M.D., Dominguez-Brauer C., Peehl D.M., Tyner A.L. (2011) "The alternative splice variant of protein tyrosine kinase 6 negatively regulates growth and enhances PTK6-mediated inhibition of beta-catenin." PLoS ONE 6:E14789-E14789