Recombinant human AXL, kinase domain, 10 µg

AXL protein kinase belongs to the TAM family comprising Tyro-3 (also called Sky), Axl, and Mer. TAM receptors are characterized by a unique structure combination of 2 immunoglobin-like domains and dual fibronectin type III repeats in the extracellular region and a cytoplasmic kinase domain. Overexpression or ectopic expression of the TAM receptors has been detected in human cancers. Abnormal expression and activation of Axl or Mer can provide a survival advantage for certain cancer cells.

Recombinant human AXL receptor tyrosine kinase, C-terminal fragment, amino acids H464-A885, recombinant and active enzyme, N-terminally fused to GST-HIS6-Thrombin cleavage site, expressed in Sf9 insect cells

Theoretical MW: 76.6 kDa (fusion protein)
Expression system: Baculovirus infected Sf9 cells
Storage buffer: 50 mM HEPES pH 7.5,100 mM NaCl, 5 mM DTT, 4 mM reduced glutathione, 20% glycerol
Protein concentration: 0.228 mg/ml (Bradford method using BSA as standard protein)
Method for determination of Km value & specific activity: Filter binding assay MSFC membrane
Specific activity: 26,000 pmol/mg x min

Entrez Gene ID: 558 
UniProtKB:  P30530

Ordering informations: shipped on dry ice

Product specific literature:

Verma A, Warner SL, Vankayalapati H, Bearss DJ, Sharma S."Targeting Axl and Mer kinases in cancer."Mol Cancer Ther. 2011 Oct;10(10):1763-73. doi: 10.1158/1535-7163.MCT-11-0116