Recombinant human CDC42 binding protein kinase beta / CDC42BPB , 10 µg

Background: CDC42BPB (CDC42 binding protein kinase beta (DMPK-like)) / Myotonic Dystrophy Kinase-Related CDC42-Binding Kinase Beta belongs to the group of serine threonine kinases. The protein contains a Cdc42/Rac-binding p21 binding domain resembling that of PAK kinase. The kinase domain of this protein is most closely related to that of myotonic dystrophy kinase-related ROK. MRCK (myotonic dystrophy kinase-related Cdc42 binding kinase), a member of the dystrophia myotonica protein kinase family that functions downstream of Cdc42, contains a C1 domain with substantial homology to that of the diacylglycerol/phorbol ester-responsive C1 domains and has been reported to bind phorbol ester.

Recombinant human CDC42BPB, (CDC42-binding protein kinase beta), Amino acids M1-H472, active enzyme, N-terminally fused to GST-HIS6-Thrombin cleavage site

Theoretical MW: 84.5 kDa (fusion protein)
Expression system: Baculovirus infected Sf9 cells
Storage buffer: 50 mM Tris-HCl, pH 8.0;100 mM NaCl, 5 mM DTT, 4 mM reduced glutathione, 20% glycerol
Protein concentration: 0,21 mg/ml (Bradford method using BSA as standard protein)
Method for determination of Km value & specific activity: Filter binding assay MSPH membrane
Specific activity: 14,000 pmol/mg x min

Entrez Gene ID: 9578 
UniProtKB: Q9Y5S2

Ordering information: shipped on dry ice

Product specific literature

Moncrieff CL1, Bailey ME, Morrison N, Johnson KJ. (1999) “Cloning and chromosomal localization of human Cdc42-binding protein kinase beta.” Genomics, 57(2):297-300.

Thompson G, Owen D, Chalk PA, Lowe PN. (1998) “Delineation of the Cdc42/Rac-binding domain of p21-activated kinase.” Biochemistry, 37(21):7885-91.

Choi SH1, Czifra G, Kedei N, Lewin NE, Lazar J, Pu Y, Marquez VE, Blumberg PM. (2008) “Characterization of the interaction of phorbol esters with the C1 domain of MRCK (myotonic dystrophy kinase-related Cdc42 binding kinase) alpha/beta.” J Biol Chem. 2008 Apr 18;283(16):