Recombinant, human CDC-like kinase 3 / CLK3 protein kinase, 10 µg

Background: CLK3 is nuclear dual-specificity kinase acting on both serine/threonine and tyrosine-containing substrates and regulates the intranuclear distribution of the serine/arginine-rich (SR) family of splicing factors. CLK3 is a member of CMGC Ser/Thr protein kinase family and Lammer subfamily and contains a non-conserved N-terminal domain

Human CLK3 (CDC-like kinase 3), full length, amino acids M1-R490 (as in NCBI/Protein entry NP_003983.2), recombinant and active enzyme, untagged, expressed in Sf9 insect cells

Theoretical MW: 59.157 kDa
Expression system: Baculovirus infected Sf9 cells
Purification: GST-Affinity Chromatography
Storage buffer: 50 mM HEPES pH 7.5,100 mM NaCl, 5 mM DTT, 20% glycerol
Protein concentration: 0.138 mg/ml (Bradford method using BSA as standard protein)
Method for determination of Km value & specific activity: Filter binding assay MSPH membrane
Specific activity: 76,000 pmol/mg x min

Entrez Gene ID: 1198
UniProtKB: P49761

Ordering information: shipped on dry ice

Product specific literature:

Duncan P.I., Stojdl D.F., Marius R.M., Scheit K.H., Bell J.C. (1998) "The Clk2 and Clk3 dual-specificity protein kinases regulate the intranuclear distribution of SR proteins and influence pre-mRNA splicing."Exp. Cell Res. 241:300-308

Eisenreich A., Bogdanov V.Y., Zakrzewicz A., Pries A., Antoniak S., Poller W., Schultheiss H.P., Rauch U. (2009) "Cdc2-like kinases and DNA topoisomerase I regulate alternative splicing of tissue factor in human endothelial cells." Circ. Res. 104:589-599

Menegay H1, Moeslein F, Landreth G.“The dual specificity protein kinase CLK3 is abundantly expressed in mature mouse spermatozoa.” Exp Cell Res.253(2):463-73.