Recombinant, human c-src tyrosine kinase (CSK) / CYL protein kinase, 10 µg

Background: CSK also known as C-Src kinase or C-terminal Src kinase is a non-receptor tyrosine-protein kinase that plays an important role in the regulation of cell growth, differentiation, migration and immune response. CSK is able to suppresses activity of the Src family of protein kinases (SFKs) by phosphorylation of tyrosine residues located in the C-terminal tails of SFKs including LCK, SRC, HCK, FYN, LYN or YES1.

Human CSK (C-Src kinase) tyrosine kinase, full length, amino acids M1-L450 (as in NCBI/Protein entry NP_004374.1), recombinant and active enzyme, N-terminal GST-HIS6 fusion protein with a Thrombin cleavage site

Theoretical MW: 80.215 kDa (fusion protein)
Expression system: Baculovirus infected Sf9 cells
Purification: One-step affinity purification using GSH-agarose
Storage buffer:  50 mM Tris-HCl, pH 8.0; 100 mM NaCl, 5 mM DTT, 15 mM reduced glutathione, 20% glycerol
Protein concentration: 0.72 mg/ml (Bradford method using BSA as standard protein)
Method for determination of Km value & specific activity: Filter binding assay MAFC membrane
Specific activity: 83,000 pmol/mg x min

Entrez Gene ID: 1445
UniProtKB: P41240

Ordering information: shipped on dry ice

Product specific literature:

Bergman M., Mustelin T., Oetken C., Partanen J., Flint N.A., Amrein K.E., Autero M., Burn P., Alitalo K. (1992) "The human p50csk tyrosine kinase phosphorylates p56lck at Tyr-505 and down regulates its catalytic activity."
EMBO J. 11:2919-2924

Sun G., Budde R.J.(1997) "Expression, purification, and initial characterization of human Yes protein tyrosine kinase from a bacterial expression system."
Arch. Biochem. Biophys. 345:135-142