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Recombinant, human vaccinia-related kinase (VRK1), 10 µg  

Recombinant, human vaccinia-related kinase (VRK1), 10 µg

Recombinant, human vaccinia-related kinase (VRK1), full lenght, amino acids M1-K396, GST-HIS6-fusion protein, expressed in Sf9 cells, active enzyme

Alternate names: vaccinia related kinase 1, PCH1, serine/threonine protein kinase VRK1

More details

PK-VRK1-A010

Availability: within 14 days

325,00 €

Background: The vaccinia-related kinase (VRK) protein family is a family of serine/threonine kinases composed of three proteins in mammals, VRK1, VRK2 and VRK3. The protein kinase VRK1 is mostly a chromatin kinase and regulates histones, but is also present in other complexes of nucleic acids, including RNA, and nucleoproteins. VRK1 is activated in response to mitogenic signals and regulates several transcription factors, including p53, ATF2 and c-Jun and histones and its covalent modifications. VRK1 is involved in regulation of cell proliferation and cell cycle regulation, e.g. induces the G1/S transition in cell cycle by promoting the expression of cyclin D1 in the G1/S phases by phosphorylating cAMP response element (CRE)-binding protein (CREB).

Recombinant human VRK1 (vaccinia related kinase 1), amino acids M1-K396, recombinant and active enzyme, N-terminally fused to GST-HIS6-Thrombin cleavage site

Theoretical MW : 75.142 kDa (fusion proteins)
Expression system: Baculovirus infected Sf9 cells
Purification: One-step affinity purification using GSH-agarose
Storage buffer: 50 mM Tris-HCl, pH 8.0; 100 mM NaCl, 5 mM DTT, 15 mM reduced glutathione, 20% glycerol
Protein concentration: 0.269 mg/ml (Bradford method using BSA as standard protein)
Method for determination of Km value & specific activity: Filter binding assay MAPH membrane
Specific activity : 30,000 pmol/mg min

Entrez Gene ID: 7443 
UniProtKB: Q99986

Ordering information: shipped on dry ice

Product specific literature

Ben Z, Gong L, Qiu Y. (2018) “High expression of VRK1 is related to poor prognosis in glioma.” Pathol Res Pract. 214(1):112-118.

Campillo-Marcos I, Lazo PA (2018) “Implication of the VRK1 chromatin kinase in the signaling responses to DNA damage: a therapeutic target?” Cell Mol Life Sci.75(13):2375-2388

Ye Seul Kim, Seong-Hoon Kim, Joon Shin, Amaravadhi Harikishore, Jong-Kwan Lim, Youngseob Jung, Ha-Na Lyu, Nam-In Baek, Kwan Yong Choi, Ho Sup Yoon, and Kyong-Tai Kim (2014) “Luteolin Suppresses Cancer Cell Proliferation by Targeting Vaccinia-Related Kinase 1” PLoS One. 9(10): e109655.