Recombinant human testis-specific protein kinase 2 (TSK2), 10 µg

Background: Testis-specific serine/threonine kinases (TSSKs) are a family of kinases in the CAM kinase family which are expressed in mammalian testis and are involved in spermiogenesis and fertilization. TSK2 has been localized to various regions of sperm, but mainly in the centrioles of post-meiotic spermatids during spermiogenesis, and it has been postulated that TSK2 has an important role in germ cell differentiation and/or sperm function. Human TSK2 phosphorylates the N-terminal region of TSKS, an endogenous substrate, and displays robust autophosphorylation. Single nucleotide polymorphisms of the TSSK2 gene are associated with impairment of spermatogenesis in idiopathically infertile males.

Recombinant human TSK2 (Testis-specific kinase 2), amino acids M1-T358, protein kinase domain, recombinant and active enzyme, N-terminally fused to GST-HIS6-Thrombin cleavage site

Purification: One-step affinity purification using GSH-agarose
Storage buffer: 50 mM Tris-HCl, pH 8.0; 100 mM NaCl, 5 mM DTT, 15 mM reduced glutathione, 20% glycerol
Protein concentration: 0.378 mg/ml (Bradford method using BSA as standard protein)
Method for determination of Km value & specific activity: Filter binding assay MSFC membrane
Specific activity : 6,000 pmol/mg min

Entrez Gene ID: 23617
UniProtKB: Q96PF2

Ordering information: shipped on dry ice

Product specific literature

Li Y, Sosnik J, Brassard L, Reese M, Spiridonov NA, Bates TC, Johnson GR, Anguita J, Visconti PE, Salicioni AM. (2011) “Expression and localization of five members of the testis-specific serine kinase (Tssk) family in mouse and human sperm and testis.” Mol Hum Reprod. 2011 Jan;17(1):42-56.

Jagathpala Shetty, Rondedrick Sinville, Igor A. Shumilin, Wladek Minor, Jianhai Zhang, Jon E. Hawkinson, Gunda I. Georg, Charles J. Flickinger, John C. Herr (2016) “Recombinant Production of Enzymatically Active Male Contraceptive Drug Target hTSSK2 - Localization of the TSKS Domain Phosphorylated by TSSK2” Protein Expr Purif.; 121: 88–96.

Hawkinson JE, Sinville R, Mudaliar D, Shetty J, Ward T, Herr JC, Georg GI (2017) “Potent Pyrimidine and Pyrrolopyrimidine Inhibitors of Testis-Specific Serine/Threonine Kinase 2 (TSSK2)”. ChemMedChem. 2017 Nov 22;12(22):1857-1865.