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Recombinant human Ephrin type-A receptor 5 (EPHA5), protein kinase domain, 10 µg  

Recombinant human Ephrin type-A receptor 5 (EPHA5), protein kinase domain, 10 µg

Recombinant human Ephrin type-A receptor 5 (EPHA5), protein kinase domain, amino acids S595-L1037, active enzyme

Alternate names: Hek7, EPH receptor A5, TYRO4, HEK7, CEK7, EPH-like kinase 7, EHK-1, Ek7, EphA5, Bsk, Ephrin type-A receptor 5, tyrosine-protein kinase receptor EHK-1, receptor protein-tyrosine kinase HEK7

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PK-EPHA5-A010

Availability: within 14 days

325,00 €

Background: EPHA5 belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. EPHA receptors binds promiscuously GPI- anchored ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. EFNA5 most probably constitutes the cognate/functional ligand for EPHA5. 

Human EPHA5 (Ephrin type-A receptor 5), amino acids S595-L1037, tyrosine kinase domain, recombinant and active enzyme, N-terminal GST-HIS6 fusion protein with a 3C cleavage site, expressed in Sf9 insect cells

Theoretical MW: 80.683 kDa (fusion protein)
Expression system: Baculovirus infected Sf9 cells
Purification: GST-Affinity Chromatography
Storage buffer:  50 mM HEPES pH 7.5, 100 mM NaCl, 5 mM DTT, 15 mM reduced glutathione, 20% glycerol
Protein concentration: 0.167 mg/ml (Bradford method using BSA as standard protein)
Method for determination of Km value & specific activity: Filter binding assay MSFC membrane
Specific activity: 247,000 pmol/mg x min

Entrez Gene ID: 2044
UniProtKB: P54756

Ordering information: shipped on dry ice

Product specific literature:

Flanagan JG1, Vanderhaeghen P. (1998) “The ephrins and Eph receptors in neural development.” Annu Rev Neurosci. 21:309-45.

Zhou R.(1997) “Regulation of topographic projection by the Eph family receptor Bsk (EphA5) and its ligands.” Cell Tissue Res. 290(2):251-9.

Akaneya Y, Sohya K, Kitamura A, Kimura F, Washburn C, Zhou R, Ninan I, Tsumoto T, Ziff EB. (2010) “Ephrin-A5 and EphA5 interaction induces synaptogenesis during early hippocampal development.” PLoS One. 5(8):e12486.