Recombinant human STK16 (serine/threonine kinase 16), TSF1, 10 µg

Background: STK16 (serine/threonine kinase 16, also known as Krct, PKL12, MPSK1, and TSF-1, TGF-beta stimulated factor 1) is a myristoylated and palmitoylated serine/threonine-protein kinase and a member of the NAK (Numb Associated Kinase) subfamily. The kinase is ubiquitously expressed with highest expression observed in the liver, testis, and kidney and conserved among all of the eukaryotes. STK16 localizes to the Golgi complex throughout the cell cycle and plays important roles in Golgi structure regulation. Thus, STK16 knockdown or kinase inhibition not only delays mitotic entry and prolongs mitosis, but also causes prometaphase and cytokinesis arrest. Furthermore, TSF1/STK16 interact with MAL2 protein, GlcNAcK kinase, and DRG1 protein, is involved in the TGF-β signaling pathway and in regulation of VEGF expression.

Recombinant Human TSF1 (TGF-beta-stimulated factor 1), full length, amino acids M1-I305 active enzyme, N-terminally fused to GST-HIS6-Thrombin cleavage site

Theoretical MW : 64.393 kDa (fusion proteins)
Expression system: Baculovirus infected Sf9 cells
Purification: One-step affinity purification using GSH-agarose
Storage buffer: 50 mM Tris-HCl, pH 8.0; 100 mM NaCl, 5 mM DTT, 15 mM reduced glutathione, 20% glycerol
Protein concentration: 1.020 mg/ml (Bradford method using BSA as standard protein)
Method for determination of Km value & specific activity: Filter binding assay MAFC membrane
Specific activity : 4.000 pmol/mg min

Entrez Gene ID: 8576
UniProtKB: O75716

Ordering information: shipped on dry ice

Product specific literature

Liu F2, Wang J, Yang X, Li B, Wu H, Qi S, Chen C, Liu X, Yu K, Wang W, Zhao Z, Wang A, Chen Y, Wang L, Gray N, Liu J, Zhang X, Liu Q (2016) “Discovery of a Highly Selective STK16 Kinase Inhibitor.” ACS Chem Biol. 17;11(6):1537-43.

Liu J, Yang X, Li B, Wang J, Wang W, Liu J, Liu Q, Zhang X. (2017) “STK16 regulates actin dynamics to control Golgi organization and cell cycle.” Sci Rep. 15;7:44607.

López-Coral A, Striz AC, Tuma PL (2018) “A Serine/Threonine Kinase 16-Based Phospho-Proteomics Screen Identifies WD Repeat Protein-1 As A Regulator Of Constitutive Secretion.” Sci Rep. 29;8(1):13049.