Recombinant human Ephrin type-A receptor 7 (EPHA7), protein kinase domain,10 µg

Background: EphA7 a widely-expressed receptor tyrosine kinase of the Eph family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. EPHA7 binds promiscuously GPI-anchored ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The kinase is involved in brain development through a caspase(CASP3)-dependent proapoptotic activity. Activation of EPHA7 results in a phosphorylation of components of the ERK signaling pathway including MAP2K1, MAP2K2, MAPK1 and MAPK3.

Human EPHA7 (Ephrin type-A receptor 7), C-terminal fragment, amino acids G579-V998 (as in
NCBI/Protein entry NP_004431.1), activated, tyrosine kinase domain, recombinant and active enzyme, N-terminal GST-HIS6 fusion protein with a 3C cleavage site, expressed in Sf9 insect cells

Theoretical MW: 78.443 kDa (fusion protein)
Expression system: Baculovirus infected Sf9 cells
Purification: GST-Affinity Chromatography
Activation: in vitro auto activation
Storage buffer:  50 mM HEPES pH 7.5, 100 mM NaCl, 5 mM DTT, 15 mM reduced glutathione, 20% glycerol
Protein concentration: 0.317 mg/ml (Bradford method using BSA as standard protein)
Method for determination of Km value & specific activity: Filter binding assay MSFC membrane
Specific activity: 21,000 pmol/mg x min

Entrez Gene ID: 2045
UniProtKB: Q15375

Ordering information: shipped on dry ice

Product specific literature:

Flanagan JG1, Vanderhaeghen P. (1998) “The ephrins and Eph receptors in neural development.” Annu Rev Neurosci. 21:309-45.

Nakanishi H., Nakamura T., Canaani E., Croce C.M. (2007) "ALL1 fusion proteins induce deregulation of EphA7 and ERK phosphorylation in human acute leukemias." Proc. Natl. Acad. Sci. U.S.A. 104:14442-14447

Clifford MA, Athar W, Leonard CE, Russo A, Sampognaro PJ, Van der Goes MS, Burton DA, Zhao X, Lalchandani RR, Sahin M, Vicini S, Donoghue MJ. (2014) “EphA7 signaling guides cortical dendritic development and spine maturation.” Proc Natl Acad Sci U S A. 111(13):4994-9.