Recombinant, human fibroblast growth factor receptor (FGF-R4), protein kinase domain, 10 µg

Background: Fibroblast growth factor receptor (FGF-R) family consists of four genes encoding closely related transmembrane, tyrosine kinase receptors (termed FGF-R1 toFGF-R4). FGF bind in the presence of heparan sulfate proteoglucans (HSPG) to FGF-R forming a dimeric 2:2:2 FGF-FGFR-HSPG ternary complex forms on the cell surface. Complex formation induces receptor dimerization and a subsequent transphosphorylation of tyrosine residues in the activation loop of the kinase domain. Receptor activation regulates many key processes such as cell proliferation, survival, migration and differentiation by linking FGF-R to RAS / MAPK and PI3K / AKT and PKC pathways. Aberrant expression and polymorphism of fibroblast growth factor receptor 4 (FGFR4) has been linked to tumor progression and anticancer drug resistance.

Human FGF-R4 (fibroblast growth factor receptor 4), amino acids R391-T802, tyrosine kinase domain, recombinant and active enzyme, N-terminally fused to GST-HIS6-Thrombin cleavage site

Theoretical MW: 75.726 kDa (fusion protein)
Expression system: Baculovirus infected Sf9 cells
Purification: One-step affinity purification using GSH-agarose
Storage buffer: 50 mM Tris-HCl, pH 8.0; 100 mM NaCl, 5 mM DTT, 4 mM reduced glutathione, 20% glycerol
Protein concentration: 0.245 mg/ml (Bradford method using BSA as standard protein)
Method for determination of Km value & specific activity: Filter binding assay MAFC membrane
Specific activity: 47,000 pmol/mg x min

Entrez Gene ID: 2264
UniProtKB: P22455

Ordering information: shipped on dry ice

Product specific literature:

Kelleher FC1, O'Sullivan H, Smyth E, McDermott R, Viterbo A."Fibroblast growth factor receptors, developmental corruption and malignant disease."Carcinogenesis. 2013 Oct;34(10):2198-205

Ron D., Reich R., Chedid M., Lengel C., Cohen O.E., Chan A.M., Neufeld G., Miki T., Tronick S.R. (1993) "Fibroblast growth factor receptor 4 is a high affinity receptor for both acidic and basic fibroblast growth factor but not for keratinocyte growth factor."J. Biol. Chem. 268:5388-5394

Sugiyama N., Varjosalo M., Meller P., Lohi J., Hyytiainen M., Kilpinen S., Kallioniemi O., Ingvarsen S., Engelholm L.H., Taipale J., Alitalo K., Keski-Oja J., Lehti K. (2010) "Fibroblast growth factor receptor 4 regulates tumor invasion by coupling fibroblast growth factor signaling to extracellular matrix degradation."Cancer Res. 70:7851-7861

Eswarakumar VP1, Lax I, Schlessinger J.(2005)"Cellular signaling by fibroblast growth factor receptors."Cytokine Growth Factor Rev. 2005 Apr;16(2):139-49.