Recombinant, human protein tyrosine kinase Fyn, GST-HIS6 Tag, 10 µg

Background: Fyn is a member of the Src family protein tyrosine kinases (SFKs) primarily localized to the cytoplasmic leaflet of the plasma membrane, where it phosphorylates tyrosine residues on key targets involved in a variety of different signaling pathways. Fyn kinase is an important mediator of mitogenic signaling and a regulator of cell cycle entry, growth and proliferation, integrin-mediated interactions, as well as cell-cell adhesion. Inhibition of Fyn, in concert with Src and Yes, resulted in inhibition of activation of PI3K/Akt/PKB and is associated with decreased cell growth. It has been shown to play a role in sensing and responding to the rigidity of extracellular matrix surfaces and is particularly upregulated in prostate cancer in contrast to the alternative members of the Src family.

Human tyrosine-protein kinase FYN (Isoform A), full length, amino acids M1-L537 (as in NCBI/Protein entry NP_002028.1), recombinant and active enzyme, N-terminal GST-HIS6 fusion protein with a Thrombin cleavage site, expressed in Sf9 insect cells

Theoretical MW: 90.358 kDa (fusion protein)
Expression system: Baculovirus infected Sf9 cells
Purification: GST-Affinity Chromatography
Storage buffer: 50 mM Tris-HCl, pH 8.0; 100 mM NaCl, 5 mM DTT, 4 mM reduced glutathione, 20% glycerol
Protein concentration: 0.093 mg/ml (Bradford method using BSA as standard protein)
Method for determination of Km value & specific activity: Filter binding assay MSFC membrane
Specific activity: 87,000 pmol/mg x min

Entrez Gene ID: 2534
UniProtKB: P06241

Ordering information: shipped on dry ice

Product specific literature:

Saito YD1, Jensen AR, Salgia R, Posadas EM.(2010) “Fyn: a novel molecular target in cancer.” Cancer.116(7):1629-37

Yadav V, Denning MF.(2011) “Fyn is induced by Ras/PI3K/Akt signaling and is required for enhanced invasion/migration.” Mol Carcinog. 50(5):346-52.