Recombinant, human hematopoietic cell kinase (HCK), active kinase, GST-HIS6 Tag, 10 µg

Background: HCK is member of the highly conserved Src family kinases belonging to the group of non-receptor tyrosine-protein kinase and is primary expressed in hematopoietic cells of the myeloid and B-lymphoid lineages. HCK plays an important role in the regulation of innate immune responses, including neutrophil, monocyte, macrophage and mast cell functions, phagocytosis, cell survival and proliferation, cell adhesion and migration. The protein kinase HCK acts downstream of FCGR1A and FCGR2A, but also CSF3R, PLAUR, the receptors for IFNG, IL2, IL6 and IL8, and integrins.

Recombinant human HCK (Hemopoietic Cell Kinase), amino acids M1-P505, recombinant and active enzyme, N-terminally fused to GST-HIS6-Thrombin cleavage site

Theoretical MW: 86.707 kDa (fusion protein)
Expression system: Baculovirus infected Sf9 cells
Purification: One-step affinity purification using GSH-agarose
Storage buffer: 50 mM HEPES pH 7.5, 100 mM NaCl, 5 mM DTT, 4 mM reduced glutathione, 20% glycerol
Protein concentration: 0.143 mg/ml (Bradford method using BSA as standard protein)
Method for determination of Km value & specific activity: Filter binding assay MSFC membrane
Specific activity: 34,000 pmol/mg x min

Entrez Gene ID: 3055
UniProtKB: P08631

Ordering information: shipped on dry ice

Product specific literature:

Taguchi T1, Kiyokawa N, Sato N, Saito M, Fujimoto J. (2000) "Characteristic expression of Hck in human B-cell precursors."Exp Hematol. 28(1):55-64.

Sicheri F, Kuriyan J.(1997) "Structures of Src-family tyrosine kinases." Curr Opin Struct Biol. 7(6):777-85

Guiet R, Poincloux R, Castandet J, Marois L, Labrousse A, Le Cabec V, Maridonneau-Parini I. (2008) "Hematopoietic cell kinase (Hck) isoforms and phagocyte duties - from signaling and actin reorganization to migration and phagocytosis."Eur J Cell Biol. 87(8-9):527-42.