Recombinant human Homeodomain-interacting protein kinase 3 (HIPK3), protein kinase domain,10 µg

Background: HIPKs (Homeodomain-interacting protein kinases) are a family of four serine/threonine kinases (HIPK1–4) highly conserved in vertebrates. HIPK3 is involved in gene transcription, cell survival, proliferation, response to DNA damage, differentiation, and development. Originally, HIPK3 was discovered by the ability to bind the neurokinin (NK) homeodomain protein. FIST/HIPK3 interacts shuttle between the cytoplasm and nuclear PML bodies in a SUMO-dependent manner. The HIPK3 kinase induced Fas-associated death domain (FADD) phosphorylation and inhibited Fas-mediated JNK activation increased splicing factor 1 (SF-1) activity for steroidogenic gene transcription in response to cAMP through phosphorylation of death-associated protein 6.

Human HIPK3 (Homeodomain-interacting protein kinase 3), internal fragment, amino acids T163-N562
(as in NCBI/Protein entry NP_005725.3), recombinant and active enzyme, activated, untagged, expressed in Sf9 insect cells

Theoretical MW: 46.163 kDa (fusion protein)
Expression system: Baculovirus infected Sf9 cells
Purification: GST-Affinity Chromatography
Storage buffer: 50 mM HEPES pH 7.5, 100 mM NaCl, 5 mM DTT, 20% glycerol
Protein concentration: 0.094 mg/ml (Bradford method using BSA as standard protein)
Method for determination of Km value & specific activity: Filter binding assay MSFC membrane
Specific activity: 12,000 pmol/mg x min

Entrez Gene ID: 10114
UniProtKB: Q9H422

Ordering information: shipped on dry ice

Product specific literature:

Rinaldo C1, Siepi F, Prodosmo A, Soddu S. (2008)"HIPKs: Jack of all trades in basic nuclear activities."Biochim Biophys Acta. 2008 Nov;1783(11):2124-9.

Shojima N1, Hara K, Fujita H, Horikoshi M, Takahashi N, Takamoto I, Ohsugi M, Aburatani H, Noda M, Kubota N, Yamauchi T, Ueki K, Kadowaki T.
(2012) "Depletion of homeodomain-interacting protein kinase 3 impairs insulin secretion and glucose tolerance in mice."Diabetologia. 2012 Dec;55(12):3318-30

KimYH,ChoiCY,LeeSJ,ContiMA,KimY(1998)" Homeodomain-interacting protein kinases, a novel family of co-repressors for homeodomain transcription factors." J Biol Chem 273:25875–25879