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Recombinant human JNK1 / MAPK8, protein kinase, 10 µg  

Recombinant human JNK1 / MAPK8, protein kinase, 10 µg

Recombinant human c-Jun N-terminal kinase 1 (JNK1), active enzyme

Alternate names: recombinant, human, MAPK8, SAPK1, JNK 1, Mitogen-activated protein kinase 8c-Jun N-terminal kinase 1Stress-activated protein kinase JNK1

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PK-JNK1-A010

Availability: within 14 days

325,00 €

Background: SAPK / JNK are members of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating a number of transcription factors, primarily components of AP-1 such as JUN, JDP2 and ATF2 and thus regulates AP-1 transcriptional activity. In T-cells, JNK1 and JNK2 are required for polarized differentiation of T-helper cells into Th1 cells.

Human JNK1 (c-Jun N-terminal kinase 1), recombinant and active enzyme, activated, untagged, expressed in E.coli

Theoretical MW: 44.292 kDa (fusion protein)
Expression system: E.coli
Purification: Immobilized Metal Affinity Chromatography
Storage buffer: 50 mM HEPES pH 7.5,100 mM NaCl, 5 mM DTT, 20% glycerol
Protein concentration: 0.513 mg/ml (Bradford method using BSA as standard protein)
Method for determination of Km value & specific activity: Filter binding assay MSFC membrane
Specific activity: 2,514,000 pmol/mg x min

Entrez Gene ID: 51528
UniProtKB: Q9P065

Ordering information: shipped on dry ice

Product specific literature

Sluss HK, Barrett T, Derijard B, Davis RJ (1994) "Signal transduction by tumor necrosis factor mediated by JNK protein kinases" Mol Cell Biol. 14(12):8376-84

Kallunki T, Su B, Tsigelny I, Sluss HK, Derijard B, Moore G, Davis R, Karin M (1994) "JNK2 contains a specificity-determining region responsible for efficient c-Jun binding and phosphorylation" Genes Dev. 8(24):2996-3007

Uciechowski P, Saklatvala J, von der Ohe J, Resch K, Szamel M, Kracht M (1996) "Interleukin 1 activates jun N-terminal kinases JNK1 and JNK2 but not extracellular regulated MAP kinase (ERK) in human glomerular mesangial cells" FEBS Lett. 394(3):273-8

Kuan CY, Yang DD, Samanta Roy DR, Davis RJ, Rakic P, Flavell RA (1999) "The Jnk1 and Jnk2 protein kinases are required for regional specific apoptosis during early brain development" Neuron. 22(4):667-76

Ham J, Eilers A, Whitfield J, Neame SJ, Shah B (2000) "c-Jun and the transcriptional control of neuronal apoptosis" Biochem. Pharmacol. 60(8):1015-21

Hochedlinger K, Wagner EF, Sabapathy K (2002) "Differential effects of JNK1 and JNK2 on signal specific induction of apoptosis" Oncogene 21(15):2441-5

Arbour N, Naniche D, Homann D, Davis RJ, Flavell RA, Oldstone MB (2002) "c-Jun NH(2)-terminal kinase (JNK)1 and JNK2 signaling pathways have divergent roles in CD8(+) T cell-mediated antiviral immunity" J Exp Med. 195(7):801-10

Bogoyevitch MA, Boehm I, Oakley A, Ketterman AJ, Barr RK (2004) "Targeting the JNK MAPK cascade for inhibition: basic science and therapeutic potential" Biochim Biophys Acta. 1697(1-2):89-101

Sabapathy K, Hochedlinger K, Nam SY, Bauer A, Karin M, Wagner EF (2004) "Distinct roles for JNK1 and JNK2 in regulating JNK activity and c-Jun-dependent cell proliferation" Mol Cell. 15(6):843-4

Tafolla E, Wang S, Wong B, Leong J, Kapila YL (2005) "JNK1 and JNK2 Oppositely Regulate p53 in Signaling Linked to Apoptosis Triggered by an Altered Fibronectin Matrix: JNK LINKS FAK AND p53" J Biol Chem. 280(20):19992-

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