Recombinant human serine/threonine-protein kinase PAK3, 10 µg

Background: The p21-activated kinases (PAKs) belong to the group of serine/threonine protein kinases and are subdivided into two groups, PAK1-3 (group I) and PAK4-6 (group II), based on sequence similarities and an autoinhibitory domain which is present in group I, but not group II PAK. Group 1 proteins are reported to be the primary effectors of the small GTPases and are activated when bound by the activated form (GTP-bound) of CDC42 and Rac1. PAKs in general have similar structural organization, including an N-terminal regulatory domain containing a p21 binding domain (PBD) and an auto-inhibitory domain (AID), and a C-terminal catalytic domain. PAK3 is the only group I member whose expression is relatively restricted to neurons whereas PAK1 is highly expressed in the nervous system but also in other tissues. PAK1 and PAK3 proteins can form heterodimers allowing a trans-inhibition of the catalytic activity of the splice variant PAK3a but not of the PAK3b coordinating PAK signaling to synchronize actin polymerization and spine stabilization. PAK3 has been implicated in the pathogenesis of intellectual disability and mutated forms of PAK3 or downregulation of PAK3 lead to morphological spine abnormalities, including an increased proportion of thin, immature spines.

Recombinant human PAK3 (p21 activated protein kinase 3), serine threonine kinase, recombinant and active enzyme, N-terminally fused to GST-HIS6-Thrombin cleavage site, expressed in Sf9 insect cells

Theoretical MW : 90.588 kDa (fusion proteins)
Expression system: Baculovirus infected Sf9 cells
Purification: One-step affinity purification usingGSH-agarose
Storage buffer: 50 mM HEPES-NaOH, pH 7.5; 100 mM NaCl, 5 mM DTT, 4 mM reduced glutathione, 20% glycerol
Protein concentration: 0.360 mg/ml (Bradford method using BSA as standard protein)
Method for determination of Km value & specific activity: Filter binding assay MSPH membrane
Specific activity : 79,000 pmol/mg min

Entrez Gene ID: 5063
UniProtKB: O75914

Ordering information: shipped on dry ice

Product specific literature

Combeau G1, Kreis P, Domenichini F, Amar M, Fossier P, Rousseau V, Barnier JV. (2012) "The p21-activated kinase PAK3 forms heterodimers with PAK1 in brain implementing trans-regulation of PAK3 activity."J Biol Chem. 2012 Aug 31;287(36):30084-96.

Dubos A1, Combeau G, Bernardinelli Y, Barnier JV, Hartley O, Gaertner H, Boda B, Muller D. (2012) "Alteration of synaptic network dynamics by the intellectual disability protein PAK3."J Neurosci. 2012 Jan 11;32(2):519-27.

Whale A, Hashim FN, Fram S, Jones GE, Wells CM. (2011) "Signalling to cancer cell invasion through PAK family kinases." Front Biosci (Landmark Ed). 1;16:849-64.