Recombinant human Lymphokine-activated killer T-cell-originated protein kinase / PBK, 10 µg

Background: PDZ binding kinase/lymphokine-activated killer T-cell –originated protein kinase (PBK/TOPK) is a 322 amino acid mitotic protein kinase and a member of the mitogen-activated protein kinase kinase (MAPKK) family. It is reported that PBK/TOPK can phosphorylate p38 and is suggested to be a novel MEK3/6-related MAPKK. PBK/TOPK is also involved in the cytokinetic function, DNA damage and repair and plays an important role in spermatogenesis. In cell cycle regulation TOPK plays a major role by acting as a substrate for cdc2/cyclin B (CDK1). Furthermore, PBK/TOPK is also expressed in hematologic tumors such as leukemia, lymphoma, and myeloma, and its expression was correlated with malignant potential of these tumors.

Recombinant human PBK (PDZ binding kinase1), amino acids M1-V322, protein kinase domain, recombinant and active enzyme, N-terminally fused to GST-HIS6-Thrombin cleavage site

Theoretical MW : 67.521 kDa (fusion proteins)
Expression system: Baculovirus infected Sf9 cells
Purification: One-step affinity purification using GSH-agarose
Storage buffer:  50 mM Tris-HCl, pH 8.0; 100 mM NaCl, 5 mM DTT, 15 mM reduced glutathione, 20% glycerol
Protein concentration: 0.256 mg/ml (Bradford method using BSA as standard protein)
Method for determination of Km value & specific activity: Filter binding assay MAFC membrane
Specific activity : 4,000 pmol/mg min

Entrez Gene ID: 55872
UniProtKB: Q96KB5

Ordering information: shipped on dry ice

Product specific literature:

Singh PK1, Srivastava AK1, Dalela D2, Rath SK3, Goel MM4, Bhatt ML5. (2014) “Expression of PDZ-binding kinase/T-LAK cell-originated protein kinase (PBK/TOPK) in human urinary bladder transitional cell carcinoma.” Immunobiology. 219(6):469-74.

Matsumoto S1, Abe Y, Fujibuchi T, Takeuchi T, Kito K, Ueda N, Shigemoto K, Gyo K.(2004) “Characterization of a MAPKK-like protein kinase TOPK.” Biochem Biophys Res Commun.325(3):997-1004.

Park JH1, Lin ML, Nishidate T, Nakamura Y, Katagiri T.(2006) „PDZ-binding kinase/T-LAK cell-originated protein kinase, a putative cancer/testis antigen with an oncogenic activity in breast cancer.” Cancer Res.;66 (18):9186-95.

Singh PK, Srivastava AK, Dalela D, Rath SK, Goel MM, Bhatt ML.(2014)” Expression of PDZ-binding kinase/T-LAK cell-originated protein kinase (PBK/TOPK) in human urinary bladder transitional cell carcinoma.” Immunobiology. 2014 Jun;219(6):469-74.