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Recombinant, human 3-phosphoinositide-dependent protein kinase 1 (PDK1), 10µg  

Recombinant, human 3-phosphoinositide-dependent protein kinase 1 (PDK1), 10µg

Recombinant human 3-phosphoinositide-dependent protein kinase 1 (PDK1), active enzyme

Alternate names: recombinant, human, protein kinase, PDK-1, PkB kinase like gene 1, 3-phosphoinositide-dependent protein kinase 1

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Availability: on stock

295,00 €

Background:  PDK1 is a constitutively active Ser/Thr kinase ubiquitously expressed in human tissues consisting of an N-terminal kinase domain and a C-terminal PH domain. Interaction of the PH domain with the PI-3K products phosphoinositol (3, 4, 5) phosphate (PIP3) and phosphoinositol (3,4) phosphate (PIP2) targets PDK1 to the plasma membrane. PDK1 acts as an upstream protein kinase phosphorylating and activating several AGC-family members (protein kinase B (PKB)/Akt, p70 ribosomal S6 kinase (S6K), p90 ribosomal S6 kinase (RSK), the serum and glucocorticoid - induced protein kinase (SGK) as well as several protein kinase C (PKC) isoforms) implicated in the control of cell growth,

Recombinant human PDK1 (3-phosphoinositide dependent protein kinase-1), amino acids M1-M460, protein kinase domain, recombinant and active enzyme, N-terminally fused to HIS6-Thrombin cleavage site, expressed in Sf9 insect cells

Theoretical MW : 56.192 kDa (fusion proteins)
Expression system: Baculovirus infected Sf9 cells
Purification: One-step affinity purification using Immobilized Metal Affinity Chromatography
Storage buffer: 50 mM HEPES pH 7.5, 100 mM NaCl, 5 mM DTT, 20% glycerol
Protein concentration: 1.223 mg/ml (Bradford method using BSA as standard protein)
Method for determination of Km value & specific activity: Filter binding assay MSPH membrane
Specific activity : 8,000 pmol/mg min

Entrez Gene ID: 5170
UniProtKB: O15530

Ordering information: shipped on dry ice

Product specific literature:

Alessi D.R., James S.R., Downes C.P., Holmes A.B., Gaffney P.R.J., Reese C.B.,Cohen P.(1997) "Characterization of a 3-phosphoinositide-dependent protein kinase which phosphorylates and activates protein kinase B alpha."Curr. Biol. 7:261-269

Sato S., Fujita N., Tsuruo T. (2002) "Regulation of kinase activity of 3-phosphoinositide-dependent protein kinase-1 by binding to 14-3-3."J. Biol. Chem. 277:39360-39367

Mora A., Komander D., van Aalten D.M., Alessi D.R.(2004) "PDK1, the master regulator of AGC kinase signal transduction."Semin. Cell Dev. Biol. 15:161-170

Bayascas J.R. (2008) "Dissecting the role of the 3-phosphoinositide-dependent protein kinase-1 (PDK1) signalling pathways." Cell Cycle 7:2978-2982