Biaffin

Newsletter

kinases protein kinase inhibitors signaling activators kinase substrates antibodies bioluminescence assays
Recombinant human neurotrophic receptor tyrosine kinase 2, TRK-B protein kinase, 10 µg  

Recombinant human neurotrophic receptor tyrosine kinase 2, TRK-B protein kinase, 10 µg

Recombinant human neurotrophic receptor tyrosine kinase 2, TRK-b, protein kinase domain, amino acids V526-G838, expressed in Sf9 insect cells, GST-HIS6 fusion protein

Alternate names: tyrosine kinase receptor B, neurotrophic tyrosine kinase, receptor, type 2, TRKB, TrkB tyrosine kinase, GP145-TrkB, BDNF-Tropomyosine Receptor Kinase B, BDNF/NT-3 Growth Factors Receptor

More details

PK-TRKB-A010

Availability: within 14 days

325,00 €

Background: Tropomyosin receptor kinase B (TrkB), encoded by NTRK2, is a neurotrophic receptor (NTRs) in the brain and is highly enriched in the neocortex, hippocampus, striatum, and brainstem, and altered TrkB expression is implicated in CNS pathologies e.g. Parkinson’s disease. The tyrosine kinase receptor TrkB, is activated specifically by brain derived growth factor and neurotrophin 4 (BDNF and NT4). BDNF binding to TrkB triggers its dimerization and autophosphorylation, resulting in activation of the three major signaling pathways involving mitogen-activated protein kinase (MAPK), phosphatidylinositol 3-kinase (PI3K), and phospholipase C-g1 (PLC-γ1), mediating neural differentiation, survival, and neurogenesis. TrkA and TrkB are known to operate as proto-oncogenes contributing to cell migration and invasion as well as neovascularization in various types of cancer.

Recombinant human TRK-B (neurotrophic tyrosine kinase, receptor, type 2 / tyrosine kinase receptor B), protein kinase domain, recombinant and active enzyme, N-terminal GST-HIS6 fusion protein with a Thrombin and 3C cleavage site, expressed in Sf9 insect cells

Theoretical MW : 66.818 kDa (fusion proteins)
Expression system: Baculovirus infected Sf9 cells
Purification: GST-Affinity Chromatography
Activation: in vitro auto-activation
Storage buffer: 50 mM HEPES pH 7.5, 100 mM NaCl, 5 mM DTT, 15 mM reduced glutathione, 20% glycerol
Protein concentration: 0.133 mg/ml (Bradford method using BSA as standard protein)
Method for determination of Km value & specific activity: Filter binding assay MSFC membrane
Specific activity : 140,000 pmol/mg min

Entrez Gene ID 4915 
UniProtKB Q16620

Ordering information: shipped on dry ice

Product specific literature

Seong Su Kang, Zhentao Zhang, Xia Liu, Fredric P. Manfredsson, Matthew J. Benskey, Xuebing Cao, Jun Xu, Yi E. Sun, Keqiang Ye (2017) “TrkB neurotrophic activities are blocked by α-synuclein, triggering dopaminergic cell death in Parkinson’s disease” Proc Natl Acad Sci U S A.; 114(40): 10773–10778.

Lange AM, Lo HW (2018) “Inhibiting TRK Proteins in Clinical Cancer Therapy.” Cancers (Basel). 10(4).

Vivek K. Gupta, Yuyi You, Veer Bala Gupta, Alexander Klistorner, Stuart L. Graham  (2013) “TrkB Receptor Signalling: Implications in Neurodegenerative, Psychiatric and Proliferative Disorders” Int J Mol Sci. 14(5): 10122–10142