Recombinant human TGF-beta receptor type-1, TGFB-R1, 10 µg

Background: TGF-β receptor types are type I receptor (TGFB-RI, TβR1 kinase or ALK5) belongs to the class of transmembrane serine/threonine protein kinases. Ligand (transforming growth factor-β, TGF-β) binding assembles a complex consisting of TβRII (signal-activating receptor) and TβR1 (signal-propagating receptor). TβRII phosphorylates TβR1, and activated kinase propagates the signal via phosphorylation of substrate SMAD proteins, SMAD2 and SMAD3. Activated SMAD protein complexes translocate to the nucleus and regulate transcriptional activation. TGF-β can also signal through Smad-independent signaling, including the PI3 kinase, MAPK, TRAF6-TAK1 and RhoA-Rock pathways e.g. by phosphorylation of ShcA proteins on tyrosine and serine. TGF-β signaling pathway has the dual role in both tumor suppression and tumor promotion and thus TGF-β is being evaluated as potential therapeutic target.

Recombinant Human Transforming growth factor, beta receptor I, protein kinase domain, recombinant and active enzyme, amino acids T200-M503, N-terminal GST-HIS6 fusion protein with a Thrombin cleavage site, expressed in Sf9 insect cells

Theoretical MW : 64.168 kDa (fusion proteins)
Expression system: Baculovirus infected Sf9 cells
Purification: GST-Affinity Chromatography
Storage buffer: 50 mM HEPES pH 7.5, 100 mM NaCl, 5 mM DTT, 15 mM reduced glutathione, 20% glycerol
Protein concentration: 0.193 mg/ml (Bradford method using BSA as standard protein)
Method for determination of Km value & specific activity: Filter binding assay MSFC membrane
Specific activity : 501,000 pmol/mg min

Entrez Gene ID 7046
UniProtKB P36897

Ordering information: shipped on dry ice

Product specific literature:

Sheen YY, Kim MJ, Park SA, Park SY, Nam JS. (2013) “Targeting the Transforming Growth Factor-β Signaling in Cancer Therapy.” Biomol Ther (Seoul). 30;21(5):323-31.

Lee M.K., Pardoux C., Hall M.C., Lee P.S., Warburton D., Qing J., Smith S.M., Derynck R. (2007) “Tgf-beta activates erk map kinase signalling through direct phosphorylation of shca.” EMBO J. 26:3957–3967.

Hendrik Ungefroren, Frank Gieseler, Roland Kaufmann, Utz Settmacher, Hendrik Lehnert, and Bernhard H. Rauch (2018) „Signaling Crosstalk of TGF-β/ALK5 and PAR2/PAR1: A Complex Regulatory Network Controlling Fibrosis and Cancer” Int J Mol Sci. 2018 Jun; 19(6): 1568.