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Human ROCK2 (Rho-associated protein kinase 2), amino acids P6-S553, protein kinase domain, recombinant and active enzyme, N-terminally fused to GST-HIS6-Thrombin cleavage site
Theoretical MW: 92.279 kDa (fusion proteins)
Expression system: Baculovirus infected Sf9 cells
Purification: One-step affinity purification using GSH-agarose
Storage buffer: 50 mM Tris-HCl, pH 8.0; 100 mM NaCl, 5 mM DTT, 4 mM reduced glutathione, 20% glycerol
Protein concentration: 0.356 mg/ml (Bradford method using BSA as standard protein)
Method for determination of Km value & specific activity: Filter binding assay MAPH membrane
Specific activity: 27,000 pmol/mg min
Entrez Gene ID: 9475
UniProtKB: O75116
Ordering informations: shipped on dry ice
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