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Recombinant human serine/threonine-protein kinase SGK3, 10 µg  

Recombinant human serine/threonine-protein kinase SGK3, 10 µg

Recombinant human serine/threonine-protein kinase SGK3, active enzyme, GST Tag

Alternate names: recombinant, human, protein kinase, SGK3, serum/glucocorticoid regulated kinase 3, SGKL

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Availability: within 7 days

465,00 €

Background: The SGK family belongs to the AGC class of kinases and consists of three members; SGK1, SGK2 and SGK3. While SGK1 and SGK3 are ubiquitously expressed in all tissues, SGK2 is primarily expressed only in liver, kidney, and pancreas. SGK expression is rapidly induced by serum and glucocorticoid. All serum/glucocorticoid-regulated kinases are activated by insulin and growth factors via phosphatidylinositol 3-kinase and the 3-phosphoinositide-dependent kinase PDK1 and shows an extensive sequence homology to Akt in the kinase domain. In contrast to SGK1 and SGK2, SGK3 contains an extra Phox homology domain that also binds PIP3 and is required for its activation and endosomal localization. SGK3 regulates several transporters, ion channels and the Na+/K+ ATPase (e.g. the voltage gated K+ channel KCNA5) and plays an important role in breast cancer metastasis by phosphorylating the E3 ubiqutin ligase AIP4 and thereby promoting the degradation of the chemokine receptor CXCR4. Furthermore, SGK3 can phosphorylate Flightless-I, a coactivator of nuclear receptors including estrogen receptor (ER) and enhance ER transactivaction.

Recombinant human protein kinase SGK3 (serum/glucocorticoid regulated kinase), amino acids M1-L496, recombinanat and active enzyme, N-terminally fused to GST, activated by PDK1

Theoretical MW : 83.206 kDa (fusion proteins)
Expression system: Baculovirus infected Sf9 cells
Purification: One-step affinity purification using GSH-agarose
Storage buffer: 50 mM Tris-HCl, pH 8.0; 100 mM NaCl, 5 mM DTT, 4 mM reduced glutathione, 20% glycerol
Protein concentration: 0.201 mg/ml (Bradford method using BSA as standard protein)
Method for determination of Km value & specific activity: Filter binding assay MSPH membrane
Specific activity : 57.000 pmol/mg min

Entrez Gene ID: 23678 
UniProtKB: Q96BR1

Ordering informations: shipped on dry ice

Product specific literature

Ahmed M1, Fezai M, Uzcategui NL, Hosseinzadeh Z, Lang F.(2016) “SGK3 Sensitivity of Voltage Gated K+ Channel Kv1.5 (KCNA5).” Cell Physiol Biochem. 2016;38(1):359-67. doi: 10.1159/000438636. Epub 2016 Jan 29.

Wang Y1, Zhou D, Phung S, Masri S, Smith D, Chen S.(2011) “SGK3 is an estrogen-inducible kinase promoting estrogen-mediated survival of breast cancer cells.” Mol Endocrinol. ;25(1):72-82. doi: 10.1210/me.2010-0294. Epub 2010 Nov 17.

Kobayashi T1, Deak M, Morrice N, Cohen P.(1999) “Characterization of the structure and regulation of two novel isoforms of serum- and glucocorticoid-induced protein kinase.” Biochem J.  15;344 Pt 1:189-97.

Tessier M1, Woodgett JR.(2006) “Role of the Phox homology domain and phosphorylation in activation of serum and glucocorticoid-regulated kinase-3.” J Biol Chem. 18;281(33):23978-89. Epub 2006 Jun 21.