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Recombinant, human neurotrophic receptor tyrosine kinase 1, TRK-A protein kinase, 10 µg  

Recombinant, human neurotrophic receptor tyrosine kinase 1, TRK-A protein kinase, 10 µg

Recombinant human neurotrophic receptor tyrosine kinase 1, TRK-A,  protein kinase domain, amino acids G443-G796, expressed in Sf9 insect cells, GST-HIS6 fusion protein

Alternate names: neurotrophic tyrosine kinase, receptor, type 1, TRK, TRKA, Trk-A, p140-TrkA, TRK1, TRK1-transforming tyrosine kinase protein

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PK-TRKA-A010

Availability: within 14 days

325,00 €

The neurotrophin receptor tropomyosin-related kinase A (TrkA) is a receptor tyrosine kinase and belongs to the neurotrophic tyrosine kinase receptor (NTKR) family, the high-affinity binding partner of nerve growth factor (NGF). The kinase regulates responses to the neurotrophins NGF and NT3 in a wide variety of normal tissues and is critical for normal development and function of both nervous and immunological systems. NGF/TRKA signaling pathway has been implicated in normal myeloid progenitor cell function, differentiation, and survival, high levels of TRKA expression have been observed in myeloid malignancies.

Recombinant, human TRK-A (neurotrophic tyrosine kinase, receptor, type 1), protein kinase domain, C-terminal  fragment, amino acids G443-G796, recombinant and active enzyme, N-terminal GST-HIS6 fusion protein with a Thrombin and 3C cleavage site, expressed in Sf9 insect cells

Theoretical MW : 69.726 kDa (fusion proteins)
Expression system: Baculovirus infected Sf9 cells
Purification: GST-Affinity Chromatography
Storage buffer: 50 mM HEPES pH 7.5, 100 mM NaCl, 5 mM DTT, 15 mM reduced glutathione, 20% glycerol
Protein concentration: 0.214 mg/ml (Bradford method using BSA as standard protein)
Method for determination of Km value & specific activity: Filter binding assay MSFC membrane
Specific activity : 100,000 pmol/mg min

Entrez Gene ID: 4914
UniProtKB: P04629

Ordering information: shipped on dry ice

Product specific literature:

Mariela Mitre, Abigail Mariga, Moses V. Chao (2017) “Neurotrophin signalling: novel insights into mechanisms and pathophysiology” Clin Sci (Lond) 1; 131(1): 13–23

Demir IE, Tieftrunk E, Schorn S, Friess H, Ceyhan GO.(2016) “Nerve growth factor & TrkA as novel therapeutic targets in cancer.” Biochim Biophys Acta. 1866(1):37-50.

Deinhardt K, Chao MV. (2014) “Trk receptors.” Handb Exp Pharmacol. 2014;220:103-19

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