Recombinant, human Casein Kinase 1 alpha1 / CK1, active protein kinase, 10 µg

The Casein kinase 1 family (EC 2.7.11.1) of protein kinases are serine/threonine-selective enzymes that function as regulators of signal transduction pathways in most eukaryotic cell types. CK1 isoforms are involved in Wnt signaling, circadian rhythms, nucleo-cytoplasmic shuttling of transcription factors, DNA repair, and DNA transcription. The CK1 family of monomeric serine–threonine protein kinases is found in eukaryotic organisms from yeast to humans. Mammals have seven family members: alpha, beta 1, gamma 1, gamma 2, gamma 3, delta, and epsilon. Isoforms range from 22 to 55 kDa and have been identified in the membranes, nucleus, and cytoplasm of eukaryotes and additionally in the mitotic spindle in mammalian cells. The family members have similar substrate specificity in vitro, and substrate selection is thought to be regulated in vivo via subcellular localization and docking sites in specific substrates.

Recombinant human CK1-alpha1, Amino acids M1-F365, N-terminally fused to GST-HIS6-Thrombin cleavage site

Theoretical MW GST-CK1: 71.4 kDa
Expression system: Baculovirus infected Sf9 cells
Purification: One-step affinity purification using glutathione agarose
Storage buffer: 50 mM Tris-HCl, pH 8.0; 100 mM NaCl, 5 mM DTT, 4 mM reduced glutathione, 20% glycerol
Storage temperature: - 80°C (avoid repeated freeze-thaw cycles!)
Shipping conditions: Dry ice
Protein concentration: 0.058 mg/ml (Bradford method using BSA as standard protein)
Method for determination of Km value & specific activity: Filter binding assay MSFC membrane
Specific activity: 2500 pmol/mg x min

Entrez Gene ID: 1452
UniProtKB: P48729

Ordering informations: shipped on dry ice

Product specific literature

Eide, Erik J.; Virshup, David M. (2001). "Casein kinase I: Another cog in the circadian clockworks". Chronobiology international 18 (3): 389–98

Cegielska, A.; Cegielska, A; Getman, ME; Landes, GM; Virshup, DM (1995). "Isolation and Characterization of Human Casein Kinase I (CKI), a Novel Member of the CKI Gene Family". Journal of Biological Chemistry 270 (25): 14875–83.

Pulgar, Victor; Marin, Oriano; Meggio, Flavio; Allende, Catherine C.; Allende, Jorge E.; Pinna, Lorenzo A. (2001). "Optimal sequences for non-phosphate-directed phoshorylation by protein kinase CK1 (casein kinase-1) – a re-evaluation". European Journal of Biochemistry 260 (2): 520–6.