Recombinant human ERK7 (MAPK15), protein kinase domain, active, Flag tag, His Tag, 10 µg

Background: ERK7 (Extracellular signal-regulated kinase also known as ERK8 or MAPK15) is a most recently identified member of the mitogen-activated protein (MAP) kinase family which is evolutionarily conserved throughout the animal kingdom. Unlike other members of the ERK family, it has constitutive activity and typical activators of other MAPKs fail to further increase ERK7's activity. ERK7 is required for proliferating cell nuclear antigen (PCNA) protein stability and, thus, for the maintenance of genomic integrity and is involved in response to amino-acid starvation and autophagy. An overexpression of ERK7 is correlated to transformation of human colon cancer cells.

Purified recombinant human ERK7/MAPK15, protein kinase domain, expressed in Baculovirus infected Sf9 cells (amino acids M1-R354) N-terminal 4xFLAG-tag  sequence,  and  C-terminal  HIS8-fusion  protein

Theoretical MW: 46.16 kDa
Expression system: Baculovirus infected Sf9 cells
Purification: Affinity purification using Immobilized Metal Affinity Chromatography
Storage buffer: 50 mM HEPES pH 7.5, 100 mM NaCl, 5 mM DTT, 20% glycerol
Protein concentration: 0.30 mg/ml (Bradford method using BSA as standard protein)
Activation: in vitro autoactivation
Method for determination of Km value & specific activity: Filter binding assay MSFC membrane
Specific activity: 44.000 pmol/mg x min (Substrate: RBER-CHKtide 200 μg / ml)

Entrez Gene ID: 225689 
UniProtKB: Q8TD08

Ordering information: shipped on dry ice

Product specific literature references:

Rossi M, Colecchia D, Ilardi G, Acunzo M, Nigita G, Sasdelli F, Celetti A, Strambi A, Staibano S, Croce CM, Chiariello M. (2016) “MAPK15 upregulation promotes cell proliferation and prevents DNA damage in male germ cell tumors.”Oncotarget. 12;7(15):20981-98

Zacharogianni M, Kondylis V, Tang Y, Farhan H, Xanthakis D, Fuchs F, Boutros M, Rabouille C.(2011) “ERK7 is a negative regulator of protein secretion in response to amino-acid starvation by modulating Sec16 membrane association.”EMBO J.30(18):3684-700.

Kuo WL, Duke CJ, Abe MK, Kaplan EL, Gomes S, Rosner MR.(2004) “ERK7 expression and kinase activity is regulated by the ubiquitin-proteosome pathway.”J Biol Chem.(22):23073-81.

Abe MK, Kahle KT, Saelzler MP, Orth K, Dixon JE, Rosner MR.(2001) “ERK7 is an autoactivated member of the MAPK family.”J Biol Chem.;276(24):21272-9.