kinases protein kinase inhibitors signaling activators kinase substrates antibodies bioluminescence assays
Recombinant human IKK beta, protein kinase 10 µg  

Recombinant human IKK beta, protein kinase 10 µg

Recombinant human I kappa B protein kinase (IKK),  beta subunit, active enzyme

Alternate names: Nuclear factor NF-kappa-B inhibitor kinase beta, Inhibitor of nuclear factor kappa-B kinase subunit beta, IKK-beta, Nuclear factor NF-kappa-B inhibitor kinase beta

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Availability: on stock

295,00 €

Background: IkappaB kinase (IKK) complex is composed of three subunits, IKK alpha, IKK eta, and IKK gamma (NEMO). While IKK alpha and IKK beta are catalytic subunits IKK gamma functions as regulatory subunit. The serine protein kinase IKK beta plays an essential role in the NF-kappa-B signaling pathway by phosphorylating inhibitors of NF-kappa-B on critical serine residues.

Human IKK-beta / Inhibitor of nuclear factor kappa-B kinase subunit beta, amino acids M1-S756, N-terminally fused to GST-HIS6-Thrombin cleavage site

Theoretical MW (IKK-beta) 120.2 kDa (fusion proteins)
Expression system: Baculovirus infected Sf9 cells
Purification: One-step affinity purification using glutathione agarose
Storage buffer: 50 mM Tris-HCl, pH 8.0;100 mM NaCl, 5 mM DTT, 4 mM reduced glutathione, 20% glycerol
Protein concentration: 0,24 mg/ml (Bradford method using BSA as standard protein)
Method for determination of Km value & specific activity: Filter binding assay MSPH membrane
Specific activity : 6,000 pmol/mg min

Entrez Gene ID: 3551
UniprotKB: O14920

Ordering information: shipped on dry ice

Product specific literature:

Li ZW1, Chu W, Hu Y, Delhase M, Deerinck T, Ellisman M, Johnson R, Karin M. (1999)"The IKKbeta subunit of IkappaB kinase (IKK) is essential for nuclear factor kappaB activation and prevention of apoptosis."J Exp Med.189(11):1839-45.

Tanaka H, Fujita N, Tsuruo T. (2005) 3-Phosphoinositide-dependent protein kinase-1-mediated IkappaB kinase beta (IkkB) phosphorylation activates NF-kappaB signaling.J Biol Chem. 280(49):40965-73.

Product Citations:

Bodur C, Kutuk O, Tezil T, Basaga H. (2012) "Inactivation of Bcl-2 through IκB kinase (IKK)-dependent phosphorylation mediates apoptosis upon exposure to 4-hydroxynonenal (HNE)." J Cell Physiol. 227(11):3556-65.

PLEASE NOTE: This product is designed for research purposes and can only be delivered to academic and business customers.