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Recombinant human serine/threonine-protein kinase NEK2, 10 µg  

Recombinant human serine/threonine-protein kinase NEK2, 10 µg

Recombinant human protein kinase NEK2 (NIMA "never in mitosis gene a"-related kinase 2), active enzyme

Alternate names: recombinant, human, protein, kinase, NimA-like protein kinase 1, Never in mitosis A-related kinase 2, NEK2A

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PK-NEK2-A010

Availability: on stock

295,00 €

Background: The NIMA-related kinases or NEKs represents a group of protein kinases whose members have key roles in mitosis. Human cells express eleven genes that encode NEK1 to NEK11 sharing approximately 40–45% identity with NIMA kinase, the founding member of this family of Aspergillus nidulan. NEK2 is a cell cycle regulated kinase with activity low in G1, increased in S and G2, and diminished after mitotic onset. It plays a direct role in enabling bipolar spindle formation through initiating the separation of centrosomes at the G2 /M transition. Furthermore, NEK2 interacts with and phosphorylates at least two components of the intercentriolar linkage, C-Nap1 and Rootletin. Interaction of NEK2 with protein phosphatase 1 (PP1) can lead to dephosphorylation and inhibition of NEK2 kinase activity.

human NEK2 (NIMA "never in mitosis gene a"-related kinase 2), amino acids M1-R445 as in GenBank entry NM_002497), N-terminally fused to GST-HIS6-Thrombin cleavage site

Theoretical MW : 82.7 kDa (fusion proteins)
Expression system: Sf9 cells
Purification: One-step affinity purification using glutathione agarose
Storage buffer: 50 mM Tris-HCl, pH 8.0; 100 mM NaCl, 5 mM DTT, 4 mM reduced glutathione, 20% glycerol
Protein concentration: 0.101 mg/ml (Bradford method using BSA as standard protein)
Method for determination of Km value & specific activity: Filter binding assay MSFC membrane
Specific activity : 22,000 pmol/mg min

Entrez Gene ID: 4751
UniProtKB: P51955

Ordering information: shipped on dry ice

Products specific literature:

O'regan L1, Blot J, Fry AM. (2007) "Mitotic regulation by NIMA-related kinases."Cell Div. 2007  Aug 29;2:25.

Rellos P1, Ivins FJ, Baxter JE, Pike A, Nott TJ, Parkinson DM, Das S, Howell S, Fedorov O, Shen QY, Fry AM, Knapp S, Smerdon SJ. (2007) "Structure and regulation of the human Nek2 centrosomal kinase."J Biol Chem. 282(9):6833-42.

Fry AM, O'Regan L, Sabir SR, Bayliss R. (2012) "Cell cycle regulation by the NEK family of protein kinases."J Cell Sci. 125(Pt 19):4423-33.

 

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