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Recombinant human Activin RIIB / ACVR2B Protein, His tag, 200µg  

Recombinant human Activin RIIB / ACVR2B Protein, His tag, 200µg

Recombinant human Activin RIIB / ACVR2B Protein, Ser19-Thr137, produced in human 293 cells (HEK293), His Tag

Synonym
Recombinant human Protein, ACVR2B, ACTRIIB, ACTR?B, ActR-IIB, ACTR-IIB, MGC116908, Activin receptor type-2B

More details

ACB-H5226-200

Availability: within 7 days

338,00 €

Background
Activin receptor type-2B (ACVR2B),is also known as ActR-IIB and MGC116908, ACVR2B is an activin type 2 receptor. Activins are dimeric growth and differentiation factors which belong to the transforming growth factor-beta (TGF-beta) superfamily of structurally related signaling proteins. Activins signal through a heteromeric complex of receptor serine kinases which include at least two type I (I and IB) and two type II (II and IIB) receptors. These receptors are all transmembrane proteins, composed of a ligand-binding extracellular domain with cysteine-rich region, a transmembrane domain, and a cytoplasmic domain with predicted serine/threonine specificity. Type I receptors are essential for signaling; and type II receptors are required for binding ligands and for expression of type I receptors. Type I and II receptors form a stable complex after ligand binding, resulting in phosphorylation of type I receptors by type II receptors. Type II receptors are considered to be constitutively active kinases. This gene encodes activin A type IIB receptor, which displays a 3- to 4-fold higher affinity for the ligand than activin A type II receptor. Defects in ACVR2B are the cause of visceral heterotaxy autosomal type 4 (HTX4).[1,2]

Source
Recombinant Human Activin RIIB, His Tag (ACB-H5226) is expressed from human 293 cells (HEK293). It contains AA Ser 19 - Thr 137 (Accession # Q13705-1).
Predicted N-terminus: Ser 19

Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 14.5 kDa. The protein migrates as 20-35 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>90% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.

Bioactivity
N / A

Clinical and Translational Updates

(1) "Hypergravity as a gravitational therapy mitigates the effects of knee osteoarthritis on the musculoskeletal system in a murine model"
Dechaumet, Cleret, Linossier et al
PLoS One (2020) 15 (12), e0243098
(2) "A Pdx-1-Regulated Soluble Factor Activates Rat and Human Islet Cell Proliferation"
Hayes, Zhang, Becker et al
Mol Cell Biol (2016) 36 (23), 2918-2930
(3) "Enhanced hyperplasia in muscles of transgenic zebrafish expressing Follistatin1"
Li, Nie, Yin et al
Sci China Life Sci (2011) 54 (2), 159-65
Showing 1-3 of 12 papers.