Recombinant human IgG1 Fc Protein, Tag free, 200µg

Background
Crystallizable fragments composed of the carboxy-terminal halves of both IMMUNOGLOBULIN HEAVY CHAINS linked to each other by disulfide bonds. Fc fragments contain the carboxy-terminal parts of the heavy chain constant regions that are responsible for the effector functions of an immunoglobulin (COMPLEMENT fixation, binding to the cell membrane via FC RECEPTORS, and placental transport). IgG1 Fc was reported has a novel role as a potential anti-inflammatory drug for treatment of human autoimmune diseases.

Source
Recombinant Human IgG Fc, Tag Free (FCC-H5214) is expressed from human 293 cells (HEK293). It contains AA Glu 99 - Lys 330 (Accession # P01857-1).
Predicted N-terminus: Glu 99

Molecular Characterization
This protein carries no "tag".
The protein has a calculated MW of 26.1 kDa. The protein migrates as 32-34 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.

Formulation
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant.

Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70^°C for 3 months under sterile conditions after reconstitution.

Bioactivity
Please refer to product data sheet.

Clinical and Translational Updates

(1) "Placental transfer dynamics and durability of maternal COVID-19 vaccine-induced antibodies in infants"
Lopez, Nziza, Chen et al
iScience (2024) 27 (3), 109273
(2) "Exploring molecular determinants and pharmacokinetic properties of IgG1-scFv bispecific antibodies"
Aertker, Pilvankar, Prass et al
MAbs (2024) 16 (1), 2318817
(3) "Distinctive antibody responses to Mycobacterium tuberculosis in pulmonary and brain infection"
Spatola, Nziza, Irvine et al
Brain (2024)
Showing 1-3 of 11794 papers.