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Recombinant human IgG1 Fc Protein, Tag free, 200µg  

Recombinant human IgG1 Fc Protein, Tag free, 200µg

Recombinant human IgG1 Fc (rhIgG1 Fc) Glu99 - Lys330, expressed in human 293 cells (HEK293), Tag free

Synonym
recombinant, human protein, Fc, IgG1 Fc, IgG Fc

More details

FCC-H5214-200

Availability: within 7 days

312,00 €

Background
Crystallizable fragments composed of the carboxy-terminal halves of both IMMUNOGLOBULIN HEAVY CHAINS linked to each other by disulfide bonds. Fc fragments contain the carboxy-terminal parts of the heavy chain constant regions that are responsible for the effector functions of an immunoglobulin (COMPLEMENT fixation, binding to the cell membrane via FC RECEPTORS, and placental transport). IgG1 Fc was reported has a novel role as a potential anti-inflammatory drug for treatment of human autoimmune diseases.(1)

Source
Recombinant human IgG1 Fc (rhIgG1 Fc) Glu99 - Lys330 (Accession # AAC82527.1) was expressed in human 293 cells (HEK293).

Molecular Characterization
rhIgG1 Fc, a 232 amino acids protein, has a calculated MW of 26 kDa. DTT-reduced protein migrates as 35 kDa protein due to glycosylation.

Endotoxin
Less than 1.0 EU per 0.5μg of the human IgG1 Fc by the LAL method.

Purity
>98% as determined by SDS-PAGE of 20 μg reduced rhIgG1 Fc. All lots are greater than 98% pure.

Formulation
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
Avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
In lyophilized state for 1 year (4C-8C); After reconstitution under sterile conditions for 1 month (4C-8C) or 3 months (-20C to -70C).

Bioactivity
Measured by its binding ability in a functional ELISA. Immobilized Human CD64, His Tag (Catalog # FCA-H52H2) at 10μg/mL (100 μL/well) can bind Human IgG Fc, Tag Free (Catalog # FCC-H5214) with a linear range of 7-41 ng/mL.

References

(1) Medinger M, Tzankov A,et al.( 2010), J Clin Immunol. 30 Suppl 1:S9-14.

Citations

Multifunctional RNA nanoparticles
Authors: Kirill A. Afonin, et al.
Journal: Nano Lett 2014
Application: As a control in Cell-based assay

Inhibition of the angiopoietin/Tie2 axis induces immunogenic modulation, which sensitizes human tumor cells to immune attack
Authors:
I Grenga, et al.
Journal:
J Immunother Cancer 2015
Application:
As a control in Cell-based assay

Frizzled proteins are colonic epithelial receptors for C. difficile toxin B
Authors:
Tao L, et al.
Journal:
Nature 2016
Application:
As a control in BLI assay

Single chain Fc-dimer-human growth hormone fusion protein for improved drug delivery

Authors:
Li Zhou, et al.
Journal:
Biomaterials 2016
Application:
As a control in Cell-based assay