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Recombinant human S100A6 Protein, Tag free, 1mg  

Recombinant human S100A6 Protein, Tag free, 1mg

Recombinant Human S100A6 Protein, Met 1 - Gly 90, expressed in E.coli cell, Tag free

Synonym
recombinant, human protein, S100A8, 60B8AG, CAGA, CFAG, CGLA, CP-10, L1Ag, MA387, MIF, MRP8, NIF, P8

More details

S16-H5111-1K

Availability: within 7 days

1 280,00 €

Background
S100 calcium binding protein A6 (S100-A6), also kown as 2A9, 5B10, CABP, CACY and PRA, is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. Most S100 proteins are disulfide-linked homodimer, and is normally present in cells derived from the neural crest, chondrocytes, macrophages, dendritic cells, etc. S100A6 may function in stimulation of Ca2+ -dependent insulin release, stimulation of prolactin secretion, and exocytosis. Chromosomal rearrangements and altered expression of this gene have been implicated in melanoma. S100A6 has been shown to interact with S100B[1-2]and SUGT1.[3]

Source
Recombinant Human S100A6 Protein (rhS100A6), Met 1 - Gly 90 (Accession # AAH01431)was expressed in E.coli cell.

Molecular Characterization
rhS100A6 contains no tag and has a calculated MW of 10.2 kDa. In DTT-reduced SDS-PAGE, rhS100A6 protein migrates as predicted.

Endotoxin
Less than 1.0 EU per μg of the rhS100A6 by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C-8°C); After reconstitution under sterile conditions for 1 month (4°C-8°C) or 3 months (-20°C to -70°C).

References

(1)Deloulme, J C, et al., 2000, J. Biol. Chem. (UNITED STATES) 275 (45): 35302–10.
(2)Yang, Q, et al., 1999, Exp. Cell Res. (UNITED STATES) 246 (2): 501–9.
(3)Nowotny, Marcin, et al., 2003, J. Biol. Chem. (United States) 278 (29): 26923–8.