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PP2C alpha, 50  µg  

PP2C alpha, 50 µg

protein phosphatase 1A/ protein phosphatase 2 C alpha

Synonyms: Protein phosphatase 1A, EC 3.1.3.16, Protein phosphatase 2C isoform alpha, PP2C-alpha, IA, PPM1A, PP2CA, MGC9201

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PP-PP2CA-050

Availability: within 3 days

195,00 €

The Ser/Thr-specific phosphatases can be divided into two major families: the PPP family, which includes PP1, PP2A, PP2B/calcineurin and the PPM family composed of PP2C. The PPM group has a strict requirement for divalent metal ions (Mg2+, Mn2+) and is insensitive to known PPP inhibitors. Mammalian PP2C was initially found to exist as two highly conserved isoforms (α and β) which show approximately 70% sequence homology. PP2Cα can be stimulated 10�15-fold by certain lipophilic compounds. By unsaturated fatty acids from lipoproteins activated PP2Cα is involved in triggering apoptosis by dephosphorylation of the pro-apoptotic protein BAD. PP2Cα also upregulates the expression and the activity of p53 and reduces the activities of CDK2 and CDK6 as well as MAPK.

Protein Informations: PPM1A/PP2Cα recombinant produced in E. coli is a single, non-glycosylated polypeptide chain containing (382 amino acids) and having a molecular mass of 46.6 kDa.Protein concentration: 1.0 mg/ml, Purity: > 95% by SDS PAGE, Specific activity: 8.000 U*/mg,

Ordering information: shipped on dry ice

PLEASE NOTE: This product is designed for research purposes and can only be delivered toacademic and business customers. 

Product specific literature:

Lu G, Wang Y. (2008) "Functional diversity of mammalian type 2C protein phosphatase isoforms: new tales from an old family." Clin Exp Pharmacol Physiol. 35(2):107-12.

Klumpp S, Thissen MC, Krieglstein J. (2006) "Protein phosphatases types 2 Calpha and 2 Cbeta in apoptosis." Biochem Soc Trans. 34(Pt 6):1370-5

Jackson MD, Denu JM. (2001) "Molecular reactions of protein phosphatases--insights from structure and chemistry." Chem Rev. 101(8):2313-40.

Lammers T, Peschke P, Ehemann V, Debus J, Slobodin B, Lavi S, Huber P. (2007) "Role of PP2Calpha in cell growth, in radio- and chemosensitivity, and in tumorigenicity." Mol Cancer. 17;6:65

Product Citations:

Mihlan S1, Reiß C, Thalheimer P, Herterich S, Gaetzner S, Kremerskothen J, Pavenstädt HJ, Lewandrowski U, Sickmann A, Butt E. (2013) "Nuclear import of LASP-1 is regulated by phosphorylation and dynamic protein-protein interactions."Oncogene. 32(16):2107-13.