H-8, 5 mg

Background: Potent inhibitor of cGMP-dependent protein kinase (PKG, IC50 ~500nM) and cAMP-dependent protein kinase (PKA, IC50 ~1200nM). Also inhibits myosin light chain kinase (MLCK).

Formula: C12H15N3O2S . 2HCl
Molecular weight: 265.3 + 73.0 g/mol
Purity: > 95%
Appearance: powder
Solubility: soluble in water and DMSO
CAS number: 113276-94-1

Also available predissolved at 10 mM in our kinase inhibitor Set (AGC group).

Product specific literature references:

Hidaka H, Inagaki M, Kawamoto S, Sasaki Y. (1984) "Isoquinolinesulfonamides, novel and potent inhibitors of cyclic nucleotide dependent protein kinase and protein kinase C." Biochemistry. 23(21):5036-41.

Hagiwara M, Inagaki M, Hidaka H. (1987) "Specific binding of a novel compound, N-[2-(methylamino)ethyl]-5-isoquinolinesulfonamide (H-8) to the active site of cAMP-dependent protein kinase." Mol Pharmacol. 31(5):523-8

Engh RA, Girod A, Kinzel V, Huber R, Bossemeyer D. (1996) "Crystal structures of catalytic subunit of cAMP-dependent protein kinase in complex with isoquinolinesulfonyl protein kinase inhibitors H7, H8, and H89. Structural implications for selectivity." J. Biol. Chem. 271(42):26157-64.

PLEASE NOTE: This product is designed for research purposes and can only be delivered to academic and business customers.