Recombinant human protein kinase C (PKC) eta, 10 µg

Background: PKCη (PKC eta) is a member of the novel PKC isozymes that regulates cell proliferation, differentiation, secretion and apoptosis. The calcium-independent, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase is primarily expressed in epithelial cells. It was reported that PKC eta distributes in the ER, the Golgi apparatus, and the nuclear envelope in epithelial cells and is constitutively present in lipid droplet (LD), an organelle made of a lipid ester core and a surface phospholipid monolayer.
The regulation of PKC eta is unique compared to other PKC isozymes. Whereas phorbol esters are potent activators of conventional and novel PKCs a persistent treatment leads to the downregulation causing termination of PKC signaling. In contrast PKCη is the only phorbol ester-sensitive PKC isozyme that resists downregulation. Furthermore, PKC eta is activated by skin epidermis uique lipids like cholesterolsulfate and suifatide, sulfated metabolites of cholesterol and cerebroside.
PKCη binds and activates the tyrosine kinase FYN in keratinocytes, which in turn blocks epidermal growth factor receptor (EGFR) signaling. In addition, PKC eta provides negative control on AKT pathway since in in MCF-7 breast adenocarcinoma cancer cells the IGF-I induced phosphorylation on Ser473 of AKT was inhibited by the PKC eta.

Recombinant human Protein kinase C (PKC) eta, recombinant and active enzyme, full length protein with N-terminal GST-tag (M1-P683), N-terminally fused to GST-HIS6-Thrombin cleavage site, expressed in Sf9 insect cells

Theoretical MW : 107.425 kDa (fusion proteins)
Expression system: Baculovirus infected Sf9 cells
Purification: One-step affinity purification using GSH-agarose
Storage buffer: 50 mM Hepes, pH 7.5; 100 mM NaCl, 5 mM DTT, 15 mM reduced glutathione, 20% glycerol
Protein concentration: 0.117 mg/ml (Bradford method using BSA as standard protein)
Method for determination of Km value & specific activity: Filter binding assay MSFC membrane
Specific activity : 71,000 pmol/mg min

Entrez Gene ID: 5583 
UniProtKB: P24723

Ordering information: shipped on dry ice

Product specific literature:

Shahaf G1, Rotem-Dai N, Koifman G, Raveh-Amit H, Frost SA, Livneh E. (2012) "PKCη is a negative regulator of AKT inhibiting the IGF-I induced proliferation."Exp Cell Res. 318(7):789-99.

Pal D1, Outram SP, Basu A. (2013) "Upregulation of PKCη by PKCε and PDK1 involves two distinct mechanisms and promotes breast cancer cell survival."Biochim Biophys Acta. 1830(8):4040-5.

Pal D, Outram SP, Basu A. (2012) "Novel regulation of protein kinase C-η."Biochem Biophys Res Commun. 425(4):836-41

Suzuki M1, Iio Y, Saito N, Fujimoto T. (2013) "Protein kinase Cη is targeted to lipid droplets."Histochem Cell Biol. 2013 Apr;139(4):505-11.

Kashiwagi M, Ohba M, Chida K, Kuroki T. (2002) "Protein kinase C eta (PKC eta): its involvement in keratinocyte differentiation." J Biochem. 132(6):853-7.