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DRB, 100 µmol  

DRB, 100 µmol

DRB is a Casein Kinase inhibitor, 5,6-Dichlorobenzimidazole riboside (DRB)

Synonyms: 5,6-Dichloro-1-b-D-ribofuranosyl benzimidazole, DRB
specific inhibitor of CK2 and CK1

More details

PKI-DRB-100

Availability: within 7 days

175,00 €

Background: DRB is a specific inhibitor of casein kinase 2 (CK2, IC50 = 6 µM) and casein kinase 1 (CK1, IC50 = 14 µM). It is also a specific inhibitor for RNA polymerase II transcription in vivo and in vitro whereat the effect on RNA polymerase II-specific transcription seems to be mediated by its inhibition of nuclear casein kinase 2. DRB is a competitive inhibitor in respect to its phosphate donor substrates ATP and GTP.

Chemical formula: C12H12Cl2N2O4
Purity: >98% (HPLC)
Molecular weight: 319.2 g/mol
Solubility: soluble in DMSO or ethanol (may require warming for complete dissolution)
Appearance: White solid
CAS number: 53-85-0

DRB, 100 µmol, ~32 mg

Product specific literature references:

Zandomeni R, Weinmann R. (1984) "Inhibitory effect of 5,6-dichloro 1-beta-D ribofuranosyl benzimidazole on a protein kinase." J. Biol. Chem. 259(23):14804-11.

Zandomeni R, Zandomeni MC, Shugar D, Weinmann R (1986) "Casein kinase type II is involved in the inhibition by 5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole of specific RNA polymerase II transcription." J. Biol. Chem. 261(7):3414-9.

Chodosh LA, Fire A, Samuels M, Sharp PA. (1989) "5,6-Dichloro 1-beta-D ribofuranosyl benzimidazole inhibits transcription elongation by RNA polymerase II in vitro." J. Biol. Chem. 264(4):2250-7

Zandomeni RO (1989) "Kinetics of inhibition by 5,6-dichloro 1-beta-D ribofuranosyl benzimidazole on calf thymus casein kinase II. "Biochem. J. 262(2):469-73.

Meggio F, Shugar D, Pinna LA (1990) "Ribofuranosyl-benzimidazole derivatives as inhibitors of casein kinase-2 and casein kinase-1." Eur. J. Biochem. 187(1):89-94

Blaydes JP, Hupp TR (1998) "DNA damage triggers DRB-resistant phosphorylation of human p53 at the CK2 site" Oncogene 17(8):1045-52

Egyhazi E, Ossoinak A, Filhol-Cochet O, Cochet C, Pigon A (1999) "The binding of the alpha subunit of protein kinase CK2 and RAP74 subunit of TFIIF to protein-coding genes in living cells is DRB sensitive" Mol. Cell. Biochem. 191(1-2):149-59

Johnston IM, Allison SJ, Morton JP, Schramm L, Scott PH, White RJ (2002) "CK2 forms a stable complex with TFIIIB and activates RNA polymerase III transcription in human cells" Mol. Cell. Biol. 22(11):3757-68

Uhle S, Medalia O, Dubiel W et al. (2003) "Protein kinase CK2 and protein kinase D are associated with the COP9 signalosome" EMBO J. 22(6):1302-12

PLEASE NOTE: This product is designed for research purposes and can only be delivered to academic and business customers.