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Background: The viral oncogene v-c-Kit was identified as the transforming gene of Hardy-Zuckerman 4 feline sarcoma virus belonging to the type III receptor tyrosine kinase closely related to PDGFR and Flt3. The ligand for c-Kit, SCF, is a bivalent protein that drives formation of c-Kit homodimers by interaction with the extracellular immunoglobulin-like domains. Ligand interaction results in intermolecular tyrosine phosphorylation of the receptor, creating docking sites for a number of Src-homology2 (SH2)-containing signal transduction molecules (GrbB2, PI3K, PLC-γ). Furthermore, c-Kit / SCF interaction induces the activation of multiple Src family members, including Src, Tec, Lyn and Fyn and the JAK/STAT pathway. By this, C-kit signaling plays a very important role in regulation of the red blood cell production, lymphocyte proliferation, mast cell development and function, melanin formation, and gamete formation.
recombinant human Mast/stem cell growth factor receptor Kit (c-KIT), C-terminal fragment, amino acids T544-V976 (as in NCBI/Protein entry NP_000213.1), N-terminally fused to GST
Theoretical MW (KIT) 77.46kDa (fusion proteins)
Expression system: Baculovirus infected Sf9 cells
Purification: One-step affinity purification using glutathione agarose
Activation: in vitro auto activation
Storage buffer: 50 mM HEPES pH 7.5, 100 mM NaCl, 5 mM DTT, 15 mM reduced glutathione, 20% glycerol
Protein concentration: 0.268 mg/ml (Bradford method using BSA as standard protein)
Method for determination of Km value & specific activity: Filter binding assay MSPH membrane
Specific activity : 63,000 pmol/mg min
Entrez Gene ID: 3815
UniProtKB: P10721
Ordering information: shipped on dry ice
Reber L1, Da Silva CA, Frossard N. (2006) Stem cell factor and its receptor c-Kit as targets for inflammatory diseases.Eur J Pharmacol. 2006 Mar 8;533(1-3):327-40.
Liang J1, Wu YL, Chen BJ, Zhang W, Tanaka Y, Sugiyama H. (2006) The C-kit receptor-mediated signal transduction and tumor-related diseases.Int J Biol Sci. 2013 May 8;9(5):435-43.
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