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Recombinant, human stem cell growth factor receptor Kit, protein kinase domain, 10 µg  

Recombinant, human stem cell growth factor receptor Kit, protein kinase domain, 10 µg

Recombinant human stem cell growth factor receptor Kit (c-KIT), proto-oncogene, protein kinase domain, amino acids T544-V976, active enzyme

Alternate names: recombinant, human, KIT, CD117, SCFR, Mast/stem cell growth factor receptor Kit, tyrosine-protein kinase Kit

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Availability: on stock

295,00 €

Background: The viral oncogene v-c-Kit was identified as the transforming gene of Hardy-Zuckerman 4 feline sarcoma virus belonging to the type III receptor tyrosine kinase closely related to PDGFR and Flt3. The ligand for c-Kit, SCF, is a bivalent protein that drives formation of c-Kit homodimers by interaction with the extracellular immunoglobulin-like domains. Ligand interaction results in intermolecular tyrosine phosphorylation of the receptor, creating docking sites for a number of Src-homology2 (SH2)-containing signal transduction molecules (GrbB2, PI3K, PLC-γ). Furthermore, c-Kit / SCF interaction induces the activation of multiple Src family members, including Src, Tec, Lyn and Fyn and the JAK/STAT pathway. By this, C-kit signaling plays a very important role in regulation of the red blood cell production, lymphocyte proliferation, mast cell development and function, melanin formation, and gamete formation.

recombinant human Mast/stem cell growth factor receptor Kit (c-KIT), amino acids T544-V976, N-terminally fused to GST 

Theoretical MW (KIT) 74.8 kDa (fusion proteins)
Expression system: Baculovirus infected Sf9 cells
Purification:     One-step affinity purification using glutathione agarose
Activation:     pre-incubation with 0.1 mM ATP, followed by (NH4)2SO4 precipitation
Multiple KIT bands probably due to different phosphorylation states
Storage buffer:     50 mM Tris-HCl, pH 8.0;100 mM NaCl, 5 mM DTT, 20% glycerol
Protein concentration: 0,258 mg/ml (Bradford method using BSA as standard protein)
Method for determination of Km value & specific activity: Filter binding assay MAFC membrane
Specific activity : 15,000 pmol/mg min

Entrez Gene ID: 3815 
UniProtKB:  P10721

Ordering information: shipped on dry ice

Product specific literature

Reber L1, Da Silva CA, Frossard N. (2006) Stem cell factor and its receptor c-Kit as targets for inflammatory diseases.Eur J Pharmacol. 2006 Mar 8;533(1-3):327-40.

Liang J1, Wu YL, Chen BJ, Zhang W, Tanaka Y, Sugiyama H. (2006) The C-kit receptor-mediated signal transduction and tumor-related diseases.Int J Biol Sci. 2013 May 8;9(5):435-43.