Recombinant human IGF1-R, protein kinase domain,10 µg

Background: IGF1-R is a receptor tyrosine kinase which mediates actions of insulin-like growth factor 1 (IGF1). Ligand binding activates the receptor kinase, leading to receptor autophosphorylation, and tyrosines phosphorylation of substrates including, the insulin-receptor substrates (IRS1/2), Shc and 14-3-3 proteins and finally to the activation of two main signaling pathways: the PI3K-AKT/PKB pathway and the Ras-MAPK pathway. IGF1R signals also through the Janus kinase/signal transducer and activator of transcription pathway (JAK/STAT) and modulates gene transcription.

Recombinant human protein kinase IGF1-R (amino acids M954-C1367, as in NCBI/Protein entry NP_000866.1), N-terminally fused to GST-HIS6-Thrombin cleavage site

Theoretical MW : 76.5 kDa (fusion proteins)
Expression system: Sf9 cells
Purification: One-step affinity purification using glutathione agarose
Storage buffer: 50 mM Tris-HCl, pH 8.0; 100 mM NaCl, 5 mM DTT, 4 mM reduced glutathione, 20% glycerol
Protein concentration: 0.183 mg/ml (Bradford method using BSA as standard protein)
Method for determination of Km value & specific activity: Filter binding assay MAFC membrane
Specific activity : 56,000 pmol/mg x min

Entrez Gene ID: 3480
UniprotKB: P08069

Ordering information: shipped on dry ice

Product specific literature:

Kato H., Faria T.N., Stannard B., Roberts C.T. Jr., LeRoith D.(1993)"Role of tyrosine kinase activity in signal transduction by the insulin-like growth factor-I (IGF-I) receptor. Characterization of kinase-deficient IGF-I receptors and the action of an IGF-I-mimetic antibody (alpha IR-3)."J. Biol. Chem. 268:2655-2661

Zong C.S., Chan J., Levy D.E., Horvath C., Sadowski H.B., Wang L.H.(2000) "Mechanism of STAT3 activation by insulin-like growth factor I receptor."J. Biol. Chem. 275:15099-15105

Hartog H., Wesseling J., Boezen H.M., van der Graaf W.T. (2007) "The insulin-like growth factor 1 receptor in cancer: old focus, new future."Eur. J. Cancer 43:1895-1904