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Recombinant, human receptor tyrosine-protein kinase ERBB4 (Her4), kinase domain, 10 µg  

Recombinant, human receptor tyrosine-protein kinase ERBB4 (Her4), kinase domain, 10 µg

Recombinant human receptor tyrosine-protein kinase erbB-4, kinase domain, amino acids, R676-V1307 active enzyme

Alternate names: ERBB-4, HER4, HER-4, MGC138404, p180erbB4

More details

PK-ERBB4-A010

Availability: on stock

295,00 €

Theroetical background: The human epidermal growth factor (EGF) receptor HER4/erbB4 is part of the erbB family of receptor tyrosine kinases, including also EGFR, HER2/erbB2 and HER3/erbB3. Activation of ErbB receptors is initiated by ligand binding, either variants of heregulins (HRGs) or different EGF related proteins, to their extracellular domain, leading to the formation of both, homo- and heterodimeric ErbB receptor complexes. In contrast to HER-3, HER-4/erbB is ativated by both, HRGs and some of the EGF ligands, providing a considerable variability in receptor activation and a diversity of HER-4 monomers and heterodimers. Upon dimerization the receptor kinase undergo autophosphorylation at specific tyrosine residues within the intracellular domain, acting as binding sites for proteins e.g. Shc, Grb2, Src, PI3K, SHP1 and SHP2.  This couples the ErbB receptor to downstream signaling pathways e.g. MAPK and the Akt pathway. ErbB4 signaling is accompanied by cleavage and release of the ErbB4 soluble intracellular domain (4ICD) which can translocate into the nucleus and associate with transcriptional regulators (translocating to the nucleus (YAP, STAT5).

Protein: human ERBB4 amino acids R676-V1307, N-terminally fused to GST-HIS6-Thrombin cleavage site

Theoretical MW : 102.1 kDa (fusion proteins)
Expression system: Baculovirus infected Sf9 cells
Specific activity : 26,000 pmol/mg x min
Method for determination of Km value & specific activity: Filter binding assay MAFC membrane
Protein concentration: 0.232 mg/ml (Bradford method using BSA as standard protein)

Entrez Gene ID: 2066
UniProtKB: P27361

Ordering information: shipped on dry ice

BIAFFIN – SERVICES:

Use the ERBB4 - SPR Binding Assay of BIAFFIN for comprehensive kinetic characterization of your small molecule kinase inhibitors.

ERbB4_Dasatinib.png

Figure: Real-time kinetic analysis of kinase inhibitor Dasatinib binding to ERbB4 using surface plasmon resonance.

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Product specific literature:

Dhomen NS, Mariadason J, Tebbutt N, Scott AM. (2012) "Therapeutic targeting of the epidermal growth factor receptor in human cancer."Crit Rev Oncog. 17(1):31-50

Veikkolainen V, Vaparanta K, Halkilahti K, Iljin K, Sundvall M, Elenius K. (2011) "Function of ERBB4 is determined by alternative splicing." Cell Cycle.10(16):2647-57.

Hollmén M, Elenius K. (2010) "Potential of ErbB4 antibodies for cancer therapy." Future Oncol. 6(1):37-53.