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Recombinant Vascular endothelial growth factor receptor 2 (VEGF-R2), protein kinase domain, 10 µg  

Recombinant Vascular endothelial growth factor receptor 2 (VEGF-R2), protein kinase domain, 10 µg

Recombinant human VEGF-R2 (vascular endothelial growth factor receptor 2), tyrosine kinase domain, amino acids D807-V1356, active enzyme, GST Tag

Alternate names: recombinant, human, protein kinase KDR, FLK1, FLK 1, VEGFR, VEGFR2, VEGF R2

More details

PK-VEGFR2-A010

Availability: on stock

295,00 €

Background: The vascular endothelial growth factor receptors (VEGFRs), primarily expressed in the vascular system, are members of the RTK superfamily belonging to the same subclass as receptors for PDGFs and FGFs. The receptors are equipped with an extracellular domain, which is organized into seven immunoglobulin (Ig)-like folds, followed by a single transmembrane region, a juxtamembrane domain, a split tyrosine-kinase domain interrupted by a 70-amino-acid kinase insert, and a C-terminal tail. Binding of VEGFs to the receptor leads to the formation of VEGFR homodimers and heterodimers. The dimerisation of the receptor facilitated an activation of the receptor-kinase by autophosphorylation on multiple tyrosine residues. Phosphorylated receptors recruit interacting proteins and thus induce the activation of several signaling pathways. VEGFR1 is required for the recruitment of haematopoietic precursors and migration of monocytes and macrophages, whereas VEGFR2 and VEGFR3 are essential for the functions of vascular endothelial and lymphendothelial cells, respectively. Upregulation of VEGF in tumor cells and its receptors (VEGFRs) on endothelial cells has been shown to contribute to tumor-associated angiogenesis.

Recombinant human protein kinase VEGF-R2, amino acids D807-V1356, N-terminally fused to GST, not activated by special procedures

Theoretical MW (VEGF-R2): 87.1 kDa (fusion proteins)
Expression system: Baculovirus infected Sf9 cells
Storage buffer: 50 mM Tris-HCl, pH 8.0; 100 mM NaCl, 5 mM DTT, 4 mM reduced glutathione, 20% glycerol
Protein concentration: 0.064 mg/ml (Bradford method using BSA as standard protein)
Method for determination of Km value & specific activity: Filter binding assay MAFC membrane
Specific activity : 20,000 pmol/mg min

Entrez Gene ID: 3791
UniProtKB: P35968

Ordering information: shipped on dry ice

Product specific literature: 

Olsson AK, Dimberg A, Kreuger J, Claesson-Welsh L.(2006) “VEGF receptor signalling - in control of vascular function.” Nat Rev Mol Cell Biol;7(5):359-71.

Christopoulos A, Ahn SM, Klein JD, Kim S.(2011) “Biology of vascular endothelial growth factor and its receptors in head and neck cancer: beyond angiogenesis.”
Head Neck.33(8):1220-9.

Roskoski R. Jr,”VEGF receptor protein- tyrosine kinases: structure and regulation”,Biochemical and Bioohysical research communications 375 (2008), 287- 291.

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