Recombinant human Insulin receptor (INS-R), protein kinase domain, 10 µg

Background: Insulin Receptor (INS-R) is a receptor tyrosine kinase which mediates the pleiotropic actions of insulin. Ligand (insulin) binding to the receptor results in a phosphorylation of several intracellular substrates, including, insulin receptor substrates (IRS1, 2, 3, 4), SHC, GAB1, CBL and other signaling intermediates leading to the activation of two main signaling pathways: the PI3K-AKT/PKB pathway, which is responsible for most of the metabolic actions of insulin, and the Ras-MAPK pathway, which regulates expression of some genes and cooperates with the PI3K pathway to control cell growth and differentiation.

Human INS-R, C-terminal fragment, amino acids G989-S1382 (as in NCBI/Protein entry NP_000199.2), N-terminally fused to GST

Theoretical MW (INS-R) 70.4 kDa (fusion proteins)
Expression system: Baculovirus infected Sf9 cells
Purification: One-step affinity purification using glutathione agarose
Storage buffer: 50 mM HEPES pH 7.5, 100 mM NaCl, 5 mM DTT, 4 mM reduced glutathione, 20% glycerol
Protein concentration: 0.189 mg/ml (Bradford method using BSA as standard protein)
Method for determination of Km value & specific activity: Filter binding assay MSFC membrane
Specific activity : 533,000 pmol/mg min

Entrez Gene ID: 3643
UniprotKB: P06213

Ordering information: shipped on dry ice

Product specific literature:

Sarfstein R, Werner H. (2013) Minireview: nuclear insulin and insulin-like growth factor-1 receptors: a novel paradigm in signal transduction.Endocrinology. 154(5):1672-9.