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Background: Fibroblast growth factor receptor (FGF-R) family consists of four genes encoding closely related transmembrane, tyrosine kinase receptors (termed FGF-R1 to FGF-R4). FGF bind in the presence of heparan sulfate proteoglucans (HSPG) to FGF-R forming a dimeric 2:2:2 FGF-FGFR-HSPG ternary complex on the cell surface. Complex formation induces receptor dimerization and a subsequent transphosphorylation of tyrosine residues in the activation loop of the kinase domain. Receptor activation regulates many key processes such as cell proliferation, survival, migration and differentiation by linking FGF-R to RAS / MAPK and PI3K / AKT and PKC pathways.
Protein: recombinant human FGF-R1 protein kinase domain (C-terminal fragment, amino acids G400-R820 ), N-terminally fused to GST-HIS6-Thrombin cleavage site
Theoretical MW : 78.1 kDa (fusion proteins)
Expression system: Sf9 cells
Purification: One-step affinity purification using glutathione agarose
Storage buffer: 50 mM Tris-HCl, pH 8.0; 100 mM NaCl, 5 mM DTT, 4 mM reduced glutathione, 20% glycerol
Protein concentration: 0.107 mg/ml (Bradford method using BSA as standard protein)
Method for determination of Km value & specific activity: Filter binding assay MAFC membrane
Specific activity : 22,000 pmol/mg x min
Entrez Gene ID: 2260
UniProtKB: P11362
Ordering information: shipped on dry ice
Figure: Real-time kinetic analysis of kinase inhibitor R-406 binding to FGF-R1 using surface plasmon resonance.
Please contact our application specialists to obtain more information and an individual quote tailored to your specific needs.
Wesche J, Haglund K, Haugsten EM. (2011) "Fibroblast growth factors and their receptors in cancer." Biochem J. 437(2):199-213
Hu Y, Bouloux PM. (2010) "Novel insights in FGFR1 regulation: lessons from Kallmann syndrome." Trends Endocrinol Metab. 21(6):385-93.
Chen GJ, Forough R. (2006) "Fibroblast growth factors, fibroblast growth factor receptors, diseases, and drugs." Recent Pat Cardiovasc Drug Discov. (2):211-24.
Recombinant human fibroblast growth factor receptor 3 (FGF-R3), protein kinase domain,10 µg - 295,00 €
8-CPT-cAMP, 100 µmol - 88,00 €
MET protein kinase inhibitor SU 11274, 1 mg - 160,00 €
FGF-1, 50 µg - 145,00 €
FGF-2, 50 µg - 145,00 €
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