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Recombinant human fibroblast growth factor receptor 3 (FGF-R3),  protein kinase domain,10 µg  

Recombinant human fibroblast growth factor receptor 3 (FGF-R3), protein kinase domain,10 µg

Human recombinant FGF-R3 (fibroblast growth factor receptor 3), protein kinase domain, amino acids E322-T806, active enzyme

Alternate names: FGFR3, ACH, CD333, CEK2, HSFGFR3EX, JTK4, FGF-R3, FGFR-3, fibroblast growth factor receptor 3

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Availability: on stock

295,00 €

Background: Fibroblast growth factor receptor (FGF-R) family consists of four genes encoding closely related transmembrane, tyrosine kinase receptors (termed FGF-R1 to FGF-R4). FGF bind in the presence of heparan sulfate proteoglucans (HSPG) to FGF-R forming a dimeric 2:2:2 FGF-FGFR-HSPG ternary complex forms on the cell surface. Complex formation induces receptor dimerization and a subsequent transphosphorylation of tyrosine residues in the activation loop of the kinase domain. Receptor activation regulates many key processes such as cell proliferation, survival, migration and differentiation by linking FGF-R to RAS / MAPK and PI3K / AKT and PKC pathways.

Human recombinant FGF-R3 (fibroblast growth factor receptor 3), active tyrosine kinase domain

Theoretical MW : 82.4 kDa (fusion proteins)
Expression system: Sf9 cells
Purification: One-step affinity purification using glutathione agarose
Storage buffer: 50 mM Tris-HCl, pH 8.0; 100 mM NaCl, 5 mM DTT, 15 mM reduced glutathione, 20% glycerol
Protein concentration: 0.103 mg/ml (Bradford method using BSA as standard protein)
Method for determination of Km value & specific activity: Filter binding assay MAFC membrane
Specific activity : 8,000 pmol/mg x min

Entrez Gene ID: 2261
UniProtKB:  P22607

Ordering information: shipped on dry ice

Product specific literature:

Lamont FR, Tomlinson DC, Cooper PA, Shnyder SD, Chester JD, Knowles MA (2010) "Small molecule FGF receptor inhibitors block FGFR-dependent urothelial carcinoma growth in vitro and in vivo" Br J Cancer 104(1):75-82

di Martino E, LHôte CG, Kennedy W, Tomlinson DC, Knowles MA (2009) "Mutant fibroblast growth factor receptor 3 induces intracellular signaling and cellular transformation in a cell type- and mutation-specific manner" Oncogene 3;28(48):4306-16

Tomlinson DC, Hurst CD, Knowles MA (2007) "Knockdown by shRNA identifies S249C mutant FGFR3 as a potential therapeutic target in bladder cancer" Oncogene 26(40):5889-99

L\'Hôte CG, Knowles MA. (2005) "Cell responses to FGFR3 signalling: growth, differentiation and apoptosis" Exp Cell Res. 304(2):417-31