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Recombinant human protein tyrosine kinase FGR, GST-Tag, His Tag,10 µg  

Recombinant human protein tyrosine kinase FGR, GST-Tag, His Tag,10 µg

Recombinant human FGR, proto-oncogene, amino acids M1-T529, active enzyme, GST-HIS6-Tag

Alternate names: recombinant, human, protein kinase, FG-R, SRC2, c-fgr, c-src2, p55c-fgr, Gardner-Rasheed feline sarcoma viral (v-fgr) oncogene homolog, FGR

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PK-FGR-A010

Availability: on stock

295,00 €

FGR is a non-receptor tyrosine-protein kinase and a member of the Src family of protein tyrosine kinases (PTKs) and acts downstream of receptors that bind the Fc region of immunoglobulins, such as MS4A2/FCER1B, FCGR2A and/or FCGR2B. Tyrosine-protein kinase Fgr functions as positive regulator of cell migration and regulates cytoskeleton reorganization via RAC1 activation. FGR phosphorylates SYK (in vitro) and promotes SYK-dependent activation of AKT1 and MAP kinase signaling.

purified human protein tyrosine kinase FGR (amino acids M1-T529 ), N-terminally fused to GST-HIS6-Thrombin cleavage site

Theoretical MW : 93.9 kDa (fusion proteins)
Expression system: Baculovirus infected Sf9 cells
Purification: One-step affinity purification using glutathione agarose
Storage buffer: 50 mM Tris-HCl, pH 8.0; 100 mM NaCl, 5 mM DTT, 4 mM reduced glutathione, 20% glycerol
Protein concentration: 0.055 mg/ml (Bradford method using BSA as standard protein)
Method for determination of Km value & specific activity: Filter binding assay MSFC membrane
Specific activity : 16,000 pmol/mg x min

Uniprot ID: P09769
Entrez Gene ID: 2268

Ordering information: shipped on dry ice

Product specific literature:

Sartor O., Moriuchi R., Sameshima J.H., Severino M., Gutkind J.S., Robbins K.C.(1992)"Diverse biologic properties imparted by the c-fgr proto-oncogene."J. Biol. Chem. 267:3460-3465

Berton G., Fumagalli L., Laudanna C., Sorio C.(1994)"Beta 2 integrin-dependent protein tyrosine phosphorylation and activation of the FGR protein tyrosine kinase in human neutrophils."J. Cell Biol. 126:1111-1121