kinases protein kinase inhibitors signaling activators kinase substrates antibodies bioluminescence assays
PMA, TPA (PKC activator), 5 mg  

PMA, TPA (PKC activator), 5 mg

Phorbol 12-myristate 13-acetate (PMA), 12-O-Tetradecanoylphorbol 13-acetate (TPA)

activates protein kinase C (PKC)

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Availability: within 14 days

185,00 €

PMA is the most commonly used phorbol ester. It binds to and activates protein kinase C (PKC) causing an extremely wide range of effects in cells and tissues. It is a very potent mouse skin tumor promoter and inhibits apoptosis induced by Fas, but induces apoptosis in HL-60 promyelocytic leukemia cells.

Molecular weight 616.8 g/mol. Solubility: Soluble in DMSO, methanol, ethanol, acetone, ether and dimethylformamide. Almost insoluble in aqueous buffers. Appearance: White to off-white solid. Purity >98%(HPLC). Formula C36H56O8. CAS Nr. 16561-29-8

Product specific literature references:

Castagna M, Takai Y, Nishizuka Y et al. (1982) "Direct activation of calcium-activated, phospholipid-dependent protein kinase by tumor-promoting phorbol esters" J Biol Chem. 257(13):7847-51

Ryves WJ, Evans AT, Olivier AR, Parker PJ, Evans FJ (1991) "Activation of the PKC-isotypes alpha, beta 1, gamma, delta and epsilon by phorbol esters of different biological activities" FEBS Lett. 288(1-2):5-9

Macfarlane DE, O\'Donnell PS (1993) "Phorbol ester induces apoptosis in HL-60 promyelocytic leukemia cells but not in HL-60 PET mutant"  Leukemia 7(11):1846-51

Schultz H, Engel K, Gaestel M (1997) "PMA-induced activation of the p42/44ERK- and p38RK-MAP kinase cascades in HL-60 cells is PKC dependent but not essential for differentiation to the macrophage-like phenotype" J Cell Physiol. 173(3):310-8

Liu WS, Heckman CA (1998) "The sevenfold way of PKC regulation" Cell Signal 10(8):529-42 [review]

Yakymovych I, Ten Dijke P, Heldin CH, Souchelnytskyi S (2001) "Regulation of Smad signaling by protein kinase C" FASEB J. 15(3):553-5

Product Citations:

Ozawa T, Takayama K, Okamoto R, Negoro R, Sakurai F, Tachibana M, Kawabata K, Mizuguchi H. (2015) "Generation of enterocyte-like cells from human induced pluripotent stem cells for drug absorption and metabolism studies in human small intestine."Sci Rep. 12;5:16479.

PLEASE NOTE: This product is designed for research purposes and can only be delivered to academic and business customers.