Recombinant human Interleukin-1 receptor-associated kinase 4 (IRAK4), protein kinase domain,10 µg

Background: IRAK4 (Interleukin-1 receptor-associated kinase 4) is a serine/threonine-protein kinase that plays a critical role in initiating innate immune response against foreign pathogens und functions especially in Toll-like receptor (TLR)–mediated signaling. Upon binding of TLR ligands the adaptor molecule MyD88 is recruited followed by a recruitment of IRAK4 and IRAK and the adaptor TRAF6 to the complex. Finally, the formed complex activate TGF-β–activated kinase 1 leading to the activation of NF-κB, c-Jun NH2-terminal kinase (JNK) and MKK6 resulting in induction of inflammatory cytokines and chemokines such as TNF-α, IL-1β, IL-6, and IL-8.

human IRAK4 C-terminal fragment, amino acids A104-S460 (as in NCBI/Protein entry NP_057207.2), N-terminally fused to GST-HIS6-Thrombin cleavage site

Theoretical MW : 69,9 kDa (fusion proteins)
Expression system: Sf9 cells
Purification: One-step affinity purification using glutathione agarose
Storage buffer: 50 mM HEPES pH 7.5, 100 mM NaCl, 5 mM DTT, 4 mM reduced glutathione, 20% glycerol
Protein concentration: 0.213 mg/ml (Bradford method using BSA as standard protein)
Method for determination of Km value & specific activity: Filter binding assay MAFC membrane
Specific activity : 1,809,000 pmol/mg x min

Entrez Gene ID: 51135
UniProtKB: Q9NWZ3

Ordering information: shipped on dry ice

Product specific literature:

Qin J1, Jiang Z, Qian Y, Casanova JL, Li X.(2004)"IRAK4 kinase activity is redundant for interleukin-1 (IL-1) receptor-associated kinase phosphorylation and IL-1 responsiveness."J Biol Chem. 279(25):26748-53

Kim TW, Staschke K, Bulek K, Yao J, Peters K, Oh KH, Vandenburg Y, Xiao H, Qian W, Hamilton T, Min B, Sen G, Gilmour R, Li X. (2007) "A critical role for IRAK4 kinase activity in Toll-like receptor-mediated innate immunity."J Exp Med. 204(5):1025-36.