Recombinant, human RAF (B-RAF) protein kinase domain, 10 µg

Background: Human B-RAF, a serine-threonine kinase which is activated by the small G-protein RAS, activates the protein kinase MEK, recombinant and active enzyme

Theoretical MW : 69.127 kDa (fusion proteins)
Expression system: Baculovirus infected Sf9 cells
Purification: One-step affinity purification using glutathione agarose
Storage buffer: 50 mM Tris-HCl, pH 8.0; 100 mM NaCl, 5 mM DTT, 4 mM reduced glutathione, 20%  glycerol
Protein concentration: 0.294 mg/ml (Bradford method using BSA as standard protein)
Method for determination of Km value & specific activity: Filter binding assay MSFC membrane
Specific activity : 78,000 pmol/mg x min

Entrez Gene ID: 673
UniProtKB:  P15056

Ordering information: shipped on dry ice

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