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Recombinant, human RAF (B-RAF) protein kinase domain, 10 µg  

Recombinant, human RAF (B-RAF) protein kinase domain, 10 µg

Recombinant human B-RAF, amino acids Gln417-His766 , protein kinase domain, active enzyme

Alternate names: recombinant, human protein, kinase domian,v-raf murine sarcoma viral oncogene homolog B1, BRAF1, B-Raf1

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Availability: on stock

345,00 €

Background: Human B-RAF, a serine-threonine kinase which is activated by the small G-protein RAS, activates the protein kinase MEK, recombinant and active enzyme

Theoretical MW : 69.127 kDa (fusion proteins)
Expression system: Baculovirus infected Sf9 cells
Purification: One-step affinity purification using glutathione agarose
Storage buffer: 50 mM Tris-HCl, pH 8.0; 100 mM NaCl, 5 mM DTT, 4 mM reduced glutathione, 20%  glycerol
Protein concentration: 0.294 mg/ml (Bradford method using BSA as standard protein)
Method for determination of Km value & specific activity: Filter binding assay MSFC membrane
Specific activity : 78,000 pmol/mg x min

Entrez Gene ID: 673
UniProtKB:  P15056

Ordering information: shipped on dry ice

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