Biaffin

Newsletter

kinases protein kinase inhibitors signaling activators kinase substrates antibodies bioluminescence assays
Recombinant human 4-1BB Protein, Fc Tag, 1mg  

Recombinant human 4-1BB Protein, Fc Tag, 1mg

Recombinant Human 4-1BB Protein, Gln 25 - Gln 186, produced in human 293 cells (HEK293), Fc Tag

Synonym
recomninant, human protein, TNFRSF9, 4-1BB, CD137, CDw137, ILA

More details

41B-H5258-1K

Availability: within 7 days

1 950,00 €

Background
4-1BB, also known as CD137, tumor necrosis factor receptor superfamily member 9 (TNFRSF9), induced by lymphocyte activation (ILA), is a co-stimulatory molecule of the tumor necrosis factor (TNF) receptor superfamily. CD137 can be expressed by activated T cells, but to a larger extent on CD8 than on CD4 T cells. In addition, CD137 expression is found on dendritic cells, follicular dendritic cells, natural killer cells, granulocytes and cells of blood vessel walls at sites of inflammation. The best characterized activity of CD137 is its costimulatory activity for activated T cells. Crosslinking of CD137 enhances T cell proliferation, IL-2 secretion survival and cytolytic activity. Further, it can enhance immune activity to eliminate tumors in mice. CD137 can enhance activation-induced T cell apoptosis when triggered by engagement of the TCR/CD3 complex. In addition, 4-1BB/4-1BBL co-stimulatory pathway has been shown to augment secondary CTL responses to several viruses, and meanwhile augment anti-tumor immunity. 4-1BB thus is a promising candidate for immunotherapy of human cancer. CD137 has been shown to interact with TRAF2.

Source
Recombinant Human 4-1BB Protein, With C-Fc Tag (rhCD137-Fc) Gln 25 - Gln 186 (Accession # NP_001552.2) was produced in human 293 cells (HEK293).

Molecular Characterization
rhCD137-Fc, fused with Fc fragment of human IgG1 at the C-terminus, has a calculated MW of 43.3 kDa. The predicted N-terminus is Gln 25. DTT-reduced Protein migrates as 60-65 kDa due to glycosylation.

Endotoxin
Less than 1.0 EU per μg of the rhCD137-Fc by the LAL method.

Purity
>97% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in 50 mM tris, 100 mM glycine, pH7.0.. Normally Mannitol or Trehalose are added as protectants before lyophilization.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
Avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C-8°C); After reconstitution under sterile conditions for 1 month (4°C-8°C) or 3 months (-20°C to -70°C).

Bioactivity 
Measured by its binding ability in a functional ELISA. Immobilized Human 4-1BB, Fc Tag (Cat. No. 41B-H5258) at 0.1 μg/mL (100 μL/well) can bind Biotinylated Human 4‑1BB Ligand, Fc Tag, Avi Tag (Cat. No. 41L-H82F9) with a linear range of 0.4-13 ng/mL (QC tested).

References

(1) Cooper D, et al., 2002, Eur. J. Immunol. 32 (2): 521–9.
(2) Jang, I K., et al., 1998, Biochem. Biophys. Res. Commun. (UNITED STATES) 242 (3): 613–20.
(3) Arch, R H., Thompson C B., 1998, Mol. Cell. Biol. (UNITED STATES) 18 (1): 558–65.

The following products could also be interesting for you: